SP Antibody

Datasheet

Code	CSB-PA356570XA01DLU
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Target Background

Product Details

Full Product Name

Rabbit anti-Drosophila melanogaster (Fruit fly) SP Polyclonal antibody

Uniprot No.

P05623

Target Names

Alternative Names

SP antibody; Acp70A antibody; PAPB antibody; CG17673Accessory gland-specific peptide 70A antibody; Paragonial peptide B antibody; Sex peptide antibody; SP antibody

Raised in

Rabbit

Species Reactivity

Drosophila melanogaster (Fruit fly)

Immunogen

Recombinant Drosophila melanogaster (Fruit fly) SP protein

Immunogen Species

Drosophila melanogaster (Fruit fly)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

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Target Background

Function

Male seminal protein which triggers short- and long-term post-mating behavioral responses (PMR) in female Drosophila. Binds initially to sperm where it is later cleaved to release an active peptide within the female reproductive tract. Signals via the sex peptide receptor (SPR) in female flies; may also act via other receptors. Moderates the activity of distinct neuronal circuitries in the female genital tract to promote specific PMRs including: enhanced ovulation, increased egg laying rate, increased feeding/foraging rate, induced antimicrobial peptide synthesis, reduced mating receptivity, reduced day-time sleep and reduced lifespan in multiple mated females.

Gene References into Functions

Molecular Functions and Cross-Species Activity of Sex-Peptide in Drosophila PMID: 26022240
sex peptide is required for normal magnitude and persistence of postmating responses PMID: 12893873
sex peptide is the major agent eliciting the short-term and the long-term postmating responses PMID: 12897240
Drosophila sex-peptide stimulates female innate immune system after mating via the Toll and Imd pathways. PMID: 16169493
Copulation strongly stimulates female food intake and Sex Peptide is the main agent controlling this behavioral paradigm. PMID: 16581515
SP acts by chemical mimicry of sugar components of the bacterial cell wall. Thus, SP may induce the immune system via pattern recognition receptors. PMID: 17922838
Data show significant variation in expression levels of the SP gene Acp70A across D. melanogaster isolines. PMID: 19640319

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Subcellular Location

Secreted.

Protein Families

Drosophila sex peptide family

Tissue Specificity

Main cells of the accessory glands of males (paragonial gland).

Database Links

KEGG: dme:Dmel_CG17673

STRING: 7227.FBpp0075572

UniGene: Dm.2898

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SP Antibody

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