Recombinant Drosophila melanogaster Accessory gland-specific peptide 70A (Acp70A)

Code CSB-EP356570DLU
MSDS
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Source E.coli
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Code CSB-EP356570DLU-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP356570DLU
MSDS
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Source Baculovirus
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Code CSB-MP356570DLU
MSDS
Size Pls inquire
Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
SP
Uniprot No.
Alternative Names
SP; Acp70A; PAPB; CG17673Accessory gland-specific peptide 70A; Paragonial peptide B; Sex peptide; SP
Species
Drosophila melanogaster (Fruit fly)
Expression Region
20-55
Target Protein Sequence
W EWPWNRKPTK FPIPSPNPRD KWCRLNLGPA WGGRC
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Male seminal protein which triggers short- and long-term post-mating behavioral responses (PMR) in female Drosophila. Binds initially to sperm where it is later cleaved to release an active peptide within the female reproductive tract. Signals via the sex peptide receptor (SPR) in female flies; may also act via other receptors. Moderates the activity of distinct neuronal circuitries in the female genital tract to promote specific PMRs including: enhanced ovulation, increased egg laying rate, increased feeding/foraging rate, induced antimicrobial peptide synthesis, reduced mating receptivity, reduced day-time sleep and reduced lifespan in multiple mated females.
Gene References into Functions
  1. Molecular Functions and Cross-Species Activity of Sex-Peptide in Drosophila PMID: 26022240
  2. sex peptide is required for normal magnitude and persistence of postmating responses PMID: 12893873
  3. sex peptide is the major agent eliciting the short-term and the long-term postmating responses PMID: 12897240
  4. Drosophila sex-peptide stimulates female innate immune system after mating via the Toll and Imd pathways. PMID: 16169493
  5. Copulation strongly stimulates female food intake and Sex Peptide is the main agent controlling this behavioral paradigm. PMID: 16581515
  6. SP acts by chemical mimicry of sugar components of the bacterial cell wall. Thus, SP may induce the immune system via pattern recognition receptors. PMID: 17922838
  7. Data show significant variation in expression levels of the SP gene Acp70A across D. melanogaster isolines. PMID: 19640319

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Subcellular Location
Secreted.
Protein Families
Drosophila sex peptide family
Tissue Specificity
Main cells of the accessory glands of males (paragonial gland).
Database Links

KEGG: dme:Dmel_CG17673

STRING: 7227.FBpp0075572

UniGene: Dm.2898

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