ABCG14 Antibody

Code CSB-PA861238XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ABCG14 Polyclonal antibody
Uniprot No.
Target Names
ABCG14
Alternative Names
ABCG14; INT211; WBC14; At1g31770; F27M3.2; F5M6.22; ABC transporter G family member 14; ABC transporter ABCG.14; AtABCG14; Protein INSENSITIVE TO TEMPERATURE 211; White-brown complex homolog protein 14; AtWBC14
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ABCG14 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Positive regulator of plant growth which acts as an efflux pump involved in the major root-to-shoot (acropetal) long-distance cytokinin (CK) transport via the xylem sap. Together with ABCG9 and ABCG11, required for vascular development by regulating lipid/sterol homeostasis. Involved in CK-dependent responses to oxidative stress such as hydrogen peroxide H(2)O(2).; (Microbial infection) Required for SNC1-mediated defense response against the virulent pathogen Pseudomonas syringae pv. tomato DC3000 by promoting the accumulation of trans-zeatin (tZ)-type cytokinins (CK) in the shoot.
Gene References into Functions
  1. ABCG14 transports Cytokinin (CK) from roots to the shoots, and the suppression of the SNC1-mediated defense response by the loss of ABCG14 is due to a deficiency of trans-zeatin (tZ)-type CK in the shoot. PMID: 28398838
  2. ABCG14, is essential for the acropetal translocation of the root-synthesized cytokinins. Knocking out AtABCG14 strongly impairs the translocation of cytokinins from roots to shoots, thereby affecting the plant's growth and development. PMID: 24513716
  3. results indicate that AtABCG14 is crucial for the translocation of cytokinin to the shoot. PMID: 24778257
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
ABC transporter superfamily, ABCG family, Eye pigment precursor importer (TC 3.A.1.204) subfamily
Tissue Specificity
Accumulates primarily in the pericycle and stelar cells of roots. Expressed in leaves, stems, flowers and siliques, and, at low levels, in roots. Accumulates in the phloem.
Database Links

KEGG: ath:AT1G31770

STRING: 3702.AT1G31770.1

UniGene: At.22580

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