Recombinant Arabidopsis thaliana ABC transporter G family member 14 (ABCG14), partial

Code CSB-YP861238DOA1
MSDS
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Source Yeast
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Code CSB-EP861238DOA1
MSDS
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Source E.coli
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Code CSB-EP861238DOA1-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP861238DOA1
MSDS
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Source Baculovirus
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Code CSB-MP861238DOA1
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ABCG14
Uniprot No.
Alternative Names
ABCG14; INT211; WBC14; At1g31770; F27M3.2; F5M6.22; ABC transporter G family member 14; ABC transporter ABCG.14; AtABCG14; Protein INSENSITIVE TO TEMPERATURE 211; White-brown complex homolog protein 14; AtWBC14
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Positive regulator of plant growth which acts as an efflux pump involved in the major root-to-shoot (acropetal) long-distance cytokinin (CK) transport via the xylem sap. Together with ABCG9 and ABCG11, required for vascular development by regulating lipid/sterol homeostasis. Involved in CK-dependent responses to oxidative stress such as hydrogen peroxide H(2)O(2).; (Microbial infection) Required for SNC1-mediated defense response against the virulent pathogen Pseudomonas syringae pv. tomato DC3000 by promoting the accumulation of trans-zeatin (tZ)-type cytokinins (CK) in the shoot.
Gene References into Functions
  1. ABCG14 transports Cytokinin (CK) from roots to the shoots, and the suppression of the SNC1-mediated defense response by the loss of ABCG14 is due to a deficiency of trans-zeatin (tZ)-type CK in the shoot. PMID: 28398838
  2. ABCG14, is essential for the acropetal translocation of the root-synthesized cytokinins. Knocking out AtABCG14 strongly impairs the translocation of cytokinins from roots to shoots, thereby affecting the plant's growth and development. PMID: 24513716
  3. results indicate that AtABCG14 is crucial for the translocation of cytokinin to the shoot. PMID: 24778257
Subcellular Location
Cell membrane; Multi-pass membrane protein.
Protein Families
ABC transporter superfamily, ABCG family, Eye pigment precursor importer (TC 3.A.1.204) subfamily
Tissue Specificity
Accumulates primarily in the pericycle and stelar cells of roots. Expressed in leaves, stems, flowers and siliques, and, at low levels, in roots. Accumulates in the phloem.
Database Links

KEGG: ath:AT1G31770

STRING: 3702.AT1G31770.1

UniGene: At.22580

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