Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ACR4 Polyclonal antibody

Uniprot No.

Q9LX29

Target Names

ACR4

Alternative Names

ACR4 antibody; At3g59420 antibody; F25L23.280Serine/threonine-protein kinase-like protein ACR4 antibody; EC 2.7.11.1 antibody; Protein CRINKLY 4 antibody; AtCR4 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ACR4 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Controls formative cell division in meristems, including root tips and lateral root initiation zones of the pericycle, in response to CLE40 signal. Acts with CLE40p peptide as a ligand-receptor pair in a signal transduction pathway, coordinating movement of the root tip and organization of cell divisions in the root meristem. Required during embryogenesis and development, probably for the differentiation of protoderm and epidermal cells. Involved in the regulation of cellular organization during the development of sepal margins and ovule integument outgrowth. Can phosphorylate ALE2.

Gene References into Functions

an intramolecular interaction can occur between the juxtamembrane and the N-terminal lobe of the kinase domain . PMID: 23567842
enzymatic and conformational properties PMID: 21294549
Arabidopsis ACR4 is present in two distinct compartments within cells. These represent protein export bodies and a population of internalized vesicles. PMID: 15772284
Study suggests that ALE2 and ACR4 function in the same process, while ALE1 has a different mode of action, and that these 3 genes play overlapping roles in positively regulating protoderm-specific gene expression and for the formation of leafy organs. PMID: 17376810
ACR4 identified as a key factor in promoting formative cell division in pericycle & constraining number of divisions once organogenesis has started; in root tip meristem, it has similar action by controlling cell proliferation in columella cell lineage PMID: 18948541

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Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endosome, multivesicular body membrane; Single-pass type I membrane protein. Note=Also localized into protein export bodies. Internalization may be involved in degradation of ACR4 for its rapid turn-over. In the epidermis, mostly expressed in the lateral and basal planes of cells.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Expressed in seedlings, floral buds, siliques, leaves, shoot apical meristems (SAM), and, to a lower extent, in roots.

Database Links

KEGG: ath:AT3G59420

STRING: 3702.AT3G59420.1

UniGene: At.28062

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ACR4 Antibody

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