Recombinant Arabidopsis thaliana Serine/threonine-protein kinase-like protein ACR4 (ACR4), partial

Code CSB-YP873386DOA
MSDS
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Source Yeast
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Code CSB-EP873386DOA
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Source E.coli
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Code CSB-EP873386DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP873386DOA
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Source Baculovirus
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Code CSB-MP873386DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ACR4
Uniprot No.
Alternative Names
ACR4; At3g59420; F25L23.280Serine/threonine-protein kinase-like protein ACR4; EC 2.7.11.1; Protein CRINKLY 4; AtCR4
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Controls formative cell division in meristems, including root tips and lateral root initiation zones of the pericycle, in response to CLE40 signal. Acts with CLE40p peptide as a ligand-receptor pair in a signal transduction pathway, coordinating movement of the root tip and organization of cell divisions in the root meristem. Required during embryogenesis and development, probably for the differentiation of protoderm and epidermal cells. Involved in the regulation of cellular organization during the development of sepal margins and ovule integument outgrowth. Can phosphorylate ALE2.
Gene References into Functions
  1. an intramolecular interaction can occur between the juxtamembrane and the N-terminal lobe of the kinase domain . PMID: 23567842
  2. enzymatic and conformational properties PMID: 21294549
  3. Arabidopsis ACR4 is present in two distinct compartments within cells. These represent protein export bodies and a population of internalized vesicles. PMID: 15772284
  4. Study suggests that ALE2 and ACR4 function in the same process, while ALE1 has a different mode of action, and that these 3 genes play overlapping roles in positively regulating protoderm-specific gene expression and for the formation of leafy organs. PMID: 17376810
  5. ACR4 identified as a key factor in promoting formative cell division in pericycle & constraining number of divisions once organogenesis has started; in root tip meristem, it has similar action by controlling cell proliferation in columella cell lineage PMID: 18948541

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Subcellular Location
Cell membrane; Single-pass type I membrane protein. Endosome, multivesicular body membrane; Single-pass type I membrane protein. Note=Also localized into protein export bodies. Internalization may be involved in degradation of ACR4 for its rapid turn-over. In the epidermis, mostly expressed in the lateral and basal planes of cells.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family
Tissue Specificity
Expressed in seedlings, floral buds, siliques, leaves, shoot apical meristems (SAM), and, to a lower extent, in roots.
Database Links

KEGG: ath:AT3G59420

STRING: 3702.AT3G59420.1

UniGene: At.28062

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