ADO2 Antibody

Code CSB-PA823824XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADO2 Polyclonal antibody
Uniprot No.
Target Names
ADO2
Alternative Names
ADO2 antibody; FKL1 antibody; LKP2 antibody; At2g18915 antibody; F19F24.11 antibody; Adagio protein 2 antibody; F-box only protein 2c antibody; FBX2c antibody; Flavin-binding kelch repeat F-box protein 1-like protein 1 antibody; FKF1-like protein 1 antibody; LOV kelch protein 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ADO2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO2) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including the transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. APRR1/TOC1 and APRR5 seem to be substrates of the SCF(ADO2) complex. ADO2 interacts with ADO3 and export it to cytoplasmic speckles, preventing the interaction of ADO3 with CDF1. Ubiquitination of ADO2 is not involved in this recruitment.
Gene References into Functions
  1. Data indicate that although cryptochrome 2 physically interacts with CIB1 in response to blue light, ZEITLUPE and LOV KELCH PROTEIN 2 are required for the function and blue-light suppression of degradation of CIB1. PMID: 24101505
  2. Both LKP2 and ZTL suppress early flowering, especially under short-day (noninductive) conditions, and that such suppression is dependent on FKF1. PMID: 21518052
  3. ZTL, FKF1, and LKP2 together regulate TOC1 and PRR5 degradation and are major contributors to determining the period of circadian oscillation and enhancing robustness. PMID: 20354196
  4. LKP2, a nuclear protein, interacted not only with itself but also with other members of the family, LKP1 and FKF1. PMID: 15310821
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
ADAGIO family
Tissue Specificity
Weakly expressed in seedlings, root tips, stems, leaves, flowers, young siliques, sepals and seeds.
Database Links

KEGG: ath:AT2G18915

STRING: 3702.AT2G18915.2

UniGene: At.19440

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