Recombinant Arabidopsis thaliana Adagio protein 2 (ADO2), partial

Code CSB-YP823824DOA
MSDS
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Source Yeast
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Code CSB-EP823824DOA
MSDS
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Source E.coli
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Code CSB-EP823824DOA-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP823824DOA
MSDS
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Source Baculovirus
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Code CSB-MP823824DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ADO2
Uniprot No.
Alternative Names
ADO2; FKL1; LKP2; At2g18915; F19F24.11; Adagio protein 2; F-box only protein 2c; FBX2c; Flavin-binding kelch repeat F-box protein 1-like protein 1; FKF1-like protein 1; LOV kelch protein 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO2) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including the transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. APRR1/TOC1 and APRR5 seem to be substrates of the SCF(ADO2) complex. ADO2 interacts with ADO3 and export it to cytoplasmic speckles, preventing the interaction of ADO3 with CDF1. Ubiquitination of ADO2 is not involved in this recruitment.
Gene References into Functions
  1. Data indicate that although cryptochrome 2 physically interacts with CIB1 in response to blue light, ZEITLUPE and LOV KELCH PROTEIN 2 are required for the function and blue-light suppression of degradation of CIB1. PMID: 24101505
  2. Both LKP2 and ZTL suppress early flowering, especially under short-day (noninductive) conditions, and that such suppression is dependent on FKF1. PMID: 21518052
  3. ZTL, FKF1, and LKP2 together regulate TOC1 and PRR5 degradation and are major contributors to determining the period of circadian oscillation and enhancing robustness. PMID: 20354196
  4. LKP2, a nuclear protein, interacted not only with itself but also with other members of the family, LKP1 and FKF1. PMID: 15310821
Subcellular Location
Nucleus. Cytoplasm.
Protein Families
ADAGIO family
Tissue Specificity
Weakly expressed in seedlings, root tips, stems, leaves, flowers, young siliques, sepals and seeds.
Database Links

KEGG: ath:AT2G18915

STRING: 3702.AT2G18915.2

UniGene: At.19440

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