Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ADO3 Polyclonal antibody

Uniprot No.

Q9C9W9

Target Names

ADO3

Alternative Names

ADO3 antibody; FKF1 antibody; At1g68050 antibody; T23K23.10Adagio protein 3 antibody; F-box only protein 2a antibody; FBX2a antibody; Flavin-binding kelch repeat F-box protein 1 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana ADO3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. Forms a complex with 'GIGANTEA' (GI) to regulate 'CONSTANS' (CO) expression. Promotes CO expression during the light period of long days by decreasing the stability of CDF1 and CDF2 and by interacting directly with the CO protein and stabilizing it. ADO3 function is mainly GI dependent. Does not act as a regulator of CDF1 transcription. The interactions of ADO1/ZTL and ADO2 with ADO3 prevent its interaction with CDF1.

Gene References into Functions

FKF1 inhibits CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-dependent CO degradation. Light-activated FKF1 has no apparent effect on COP1 stability but can interact with and negatively regulate COP1. We show that FKF1 can inhibit COP1 homo-dimerization. Mutation of the coiled-coil domain in COP1, which prevents dimer formation, impairs COP1 function in coordinating flowering time. PMID: 29273730
study demonstrates that FKF1 protein stabilizes CONSTANS (CO)protein in the afternoon in long days; together with CO transcriptional regulation, FKF1 protein controls robust FLOWERING LOCUS T FT) mRNA induction through multiple feedforward mechanisms that accurately control flowering timing PMID: 22628657
This result indicates that the conformational change in the loop region represents a major change in the FKF1- light-oxygen-voltage domain photoreaction. PMID: 21112308
Quaternary structure of FKF1 protein PMID: 15710392
Photoreaction of the FKF1-LOV(LOV-voltage sensing domain binding a flavin mononucleotide)polypeptide was studied by low-temperature absorption spectroscopy PMID: 16953568

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Subcellular Location

Nucleus. Cytoplasm. Note=Moves from the nucleus into cytosolic speckles upon interaction with ADO1 and ADO2.

Protein Families

ADAGIO family

Tissue Specificity

Highly expressed in stomata and leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments.

Database Links

KEGG: ath:AT1G68050

STRING: 3702.AT1G68050.1

UniGene: At.20568

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ADO3 Antibody

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