Recombinant Arabidopsis thaliana Adagio protein 3 (ADO3), partial

Code CSB-YP883722DOA
MSDS
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Source Yeast
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Code CSB-EP883722DOA
MSDS
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Source E.coli
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Code CSB-EP883722DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP883722DOA
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Source Baculovirus
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Code CSB-MP883722DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ADO3
Uniprot No.
Alternative Names
ADO3; FKF1; At1g68050; T23K23.10Adagio protein 3; F-box only protein 2a; FBX2a; Flavin-binding kelch repeat F-box protein 1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Component of an E3 ubiquitin ligase complex that plays a central role in blue light-dependent circadian cycles. Acts as a blue light photoreceptor, due to the presence of FMN, that mediates light-regulated protein degradation of critical clock components by targeting them to the proteasome complex. The SCF(ADO3) E3 ubiquitin ligase complex is involved in the regulation of circadian clock-dependent processes including transition to flowering time, hypocotyl elongation, cotyledons and leaf movement rhythms. Forms a complex with 'GIGANTEA' (GI) to regulate 'CONSTANS' (CO) expression. Promotes CO expression during the light period of long days by decreasing the stability of CDF1 and CDF2 and by interacting directly with the CO protein and stabilizing it. ADO3 function is mainly GI dependent. Does not act as a regulator of CDF1 transcription. The interactions of ADO1/ZTL and ADO2 with ADO3 prevent its interaction with CDF1.
Gene References into Functions
  1. FKF1 inhibits CONSTITUTIVE PHOTOMORPHOGENIC1 (COP1)-dependent CO degradation. Light-activated FKF1 has no apparent effect on COP1 stability but can interact with and negatively regulate COP1. We show that FKF1 can inhibit COP1 homo-dimerization. Mutation of the coiled-coil domain in COP1, which prevents dimer formation, impairs COP1 function in coordinating flowering time. PMID: 29273730
  2. study demonstrates that FKF1 protein stabilizes CONSTANS (CO)protein in the afternoon in long days; together with CO transcriptional regulation, FKF1 protein controls robust FLOWERING LOCUS T FT) mRNA induction through multiple feedforward mechanisms that accurately control flowering timing PMID: 22628657
  3. This result indicates that the conformational change in the loop region represents a major change in the FKF1- light-oxygen-voltage domain photoreaction. PMID: 21112308
  4. Quaternary structure of FKF1 protein PMID: 15710392
  5. Photoreaction of the FKF1-LOV(LOV-voltage sensing domain binding a flavin mononucleotide)polypeptide was studied by low-temperature absorption spectroscopy PMID: 16953568

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Subcellular Location
Nucleus. Cytoplasm. Note=Moves from the nucleus into cytosolic speckles upon interaction with ADO1 and ADO2.
Protein Families
ADAGIO family
Tissue Specificity
Highly expressed in stomata and leaves and to a lower extent in seeds, roots, rosettes, stems and siliques. Also present in sepals and anther filaments.
Database Links

KEGG: ath:AT1G68050

STRING: 3702.AT1G68050.1

UniGene: At.20568

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