AFT2 Antibody

Code CSB-PA782524XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) AFT2 Polyclonal antibody
Uniprot No.
Target Names
AFT2
Alternative Names
AFT2 antibody; YPL202C antibody; Iron-regulated transcriptional activator AFT2 antibody; Activator of iron transcription protein 2 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) AFT2 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription factor required for iron homeostasis and resistance to oxidative stress. With RCS1, activates the gene expression in response to low-iron conditions, also called iron regulon.
Gene References into Functions
  1. the absence of Aft2 leads to enhanced Pho90 function, involving both Spl2-dependent and independent events and resulting in selenite hyperaccumulation and toxicity. PMID: 27618952
  2. Two Aft2 monomers bind a [2Fe-2S] cluster (or Fe2+) through a Cys-Asp-Cys motif, leading to dimerization of Aft2 and decreased DNA-binding affinity. PMID: 24591629
  3. Aft2 activates the transcription of genes involved in intracellular iron use in the absence of Aft1. PMID: 16024809
Subcellular Location
Nucleus.
Database Links

KEGG: sce:YPL202C

STRING: 4932.YPL202C

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