AGD3 Antibody

Code CSB-PA722803XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGD3 Polyclonal antibody
Uniprot No.
Target Names
AGD3
Alternative Names
AGD3 antibody; FKD2 antibody; SFC antibody; VAN3 antibody; At5g13300 antibody; T31B5.120ADP-ribosylation factor GTPase-activating protein AGD3 antibody; ARF GAP AGD3 antibody; Protein ARF-GAP DOMAIN 3 antibody; AtAGD3 antibody; Protein FORKED 2 antibody; Protein SCARFACE antibody; Protein VASCULAR NETWORK 3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AGD3 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Involved in the spatial control of provascular differentiation. Required for the formation of the normal pattern of continuous secondary veins. Involved in auxin signaling but not in polar auxin transport or in auxin responses. Required for PIN1 internalization in roots.
Gene References into Functions
  1. Data show that ARF-GEF GNOM and ARF-GAP vascular network defective 3 (VAN3), both of which are involved in polar auxin transport-dependent morphogenesis, localize at the plasma membranes as well as in intracellular structures. PMID: 21118984
  2. expression of this gene is induced by auxin and positively regulated by VAN3 itself (VAN3) PMID: 15743878
  3. Scarface (SFC) is required for normal intracellular transport of PIN1 from the plasma membrane to the endosome. [Scarface] [SFC] PMID: 16698946
  4. existence of PtdIns(4)P and/or IP(3)-dependent subcellular targeting and regulation of VAN3 ACAP activity that governs plant vascular tissue continuity PMID: 19363154
  5. VAN3 is involved in vesicle trafficking and DRP1A is responsible for the vascular formation synergistically working with the VAN3 protein. [VAN3] PMID: 15923323

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Subcellular Location
Golgi apparatus, trans-Golgi network.
Tissue Specificity
Broadly expressed. Detected in developing veins of the leaf and root. Detected in roots, hypocotyls, cotyledons, leaves, siliques and shoot apical meristems.
Database Links

KEGG: ath:AT5G13300

STRING: 3702.AT5G13300.1

UniGene: At.32090

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