Recombinant Arabidopsis thaliana ADP-ribosylation factor GTPase-activating protein AGD3 (AGD3), partial

Code CSB-YP722803DOA
MSDS
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Source Yeast
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Code CSB-EP722803DOA
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Source E.coli
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Code CSB-EP722803DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP722803DOA
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Source Baculovirus
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Code CSB-MP722803DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGD3
Uniprot No.
Alternative Names
AGD3; FKD2; SFC; VAN3; At5g13300; T31B5.120ADP-ribosylation factor GTPase-activating protein AGD3; ARF GAP AGD3; Protein ARF-GAP DOMAIN 3; AtAGD3; Protein FORKED 2; Protein SCARFACE; Protein VASCULAR NETWORK 3
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Involved in the spatial control of provascular differentiation. Required for the formation of the normal pattern of continuous secondary veins. Involved in auxin signaling but not in polar auxin transport or in auxin responses. Required for PIN1 internalization in roots.
Gene References into Functions
  1. Data show that ARF-GEF GNOM and ARF-GAP vascular network defective 3 (VAN3), both of which are involved in polar auxin transport-dependent morphogenesis, localize at the plasma membranes as well as in intracellular structures. PMID: 21118984
  2. expression of this gene is induced by auxin and positively regulated by VAN3 itself (VAN3) PMID: 15743878
  3. Scarface (SFC) is required for normal intracellular transport of PIN1 from the plasma membrane to the endosome. [Scarface] [SFC] PMID: 16698946
  4. existence of PtdIns(4)P and/or IP(3)-dependent subcellular targeting and regulation of VAN3 ACAP activity that governs plant vascular tissue continuity PMID: 19363154
  5. VAN3 is involved in vesicle trafficking and DRP1A is responsible for the vascular formation synergistically working with the VAN3 protein. [VAN3] PMID: 15923323

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Subcellular Location
Golgi apparatus, trans-Golgi network.
Tissue Specificity
Broadly expressed. Detected in developing veins of the leaf and root. Detected in roots, hypocotyls, cotyledons, leaves, siliques and shoot apical meristems.
Database Links

KEGG: ath:AT5G13300

STRING: 3702.AT5G13300.1

UniGene: At.32090

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