AGD5 Antibody

Code CSB-PA880782XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGD5 Polyclonal antibody
Uniprot No.
Target Names
AGD5
Alternative Names
AGD5 antibody; MTV4 antibody; NEV antibody; ZIG3 antibody; ZIGA3 antibody; At5g54310 antibody; MDK4.13ADP-ribosylation factor GTPase-activating protein AGD5 antibody; ARF GAP AGD5 antibody; Protein ARF-GAP DOMAIN 5 antibody; AtAGD5 antibody; Protein MODIFIED TRANSPORT TO THE VACUOLE 4 antibody; Protein NEVERSHED antibody; Protein ZIGA3 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AGD5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Mediates clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network (TGN). Promotes plant growth. Involved in the regulation of membrane trafficking and cell separation during floral organ shedding and abscission. Prevents abscission zone (AZ) cells enlargement. Exhibits ARF-GTPase activity toward ARF1 at TGN.
Gene References into Functions
  1. IDA and NEV function during both the cell-wall loosening and the cell separation stages of the abscission process. In addition, NEV acts to inhibit premature cell expansion. PMID: 23963677
  2. AGD5, a a GTPase-activating protein, is distributed on a sub-population of the trans-Golgi network, where it co-localises with ARF1. PMID: 21105926
  3. NEV ARF-GAP activity at the trans-Golgi network and distinct endosomal compartments is required for the proper trafficking of cargo molecules required for cell separation. [NEV] PMID: 19429787
Subcellular Location
Golgi apparatus, trans-Golgi network. Endosome. Cytoplasmic vesicle, clathrin-coated vesicle.
Tissue Specificity
Expressed in inflorescence stems, abscission zones, stigmas, roots, roots meristems, embryos, and floral and leaf vasculatures.
Database Links

KEGG: ath:AT5G54310

STRING: 3702.AT5G54310.1

UniGene: At.20358

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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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