Recombinant Arabidopsis thaliana Probable ADP-ribosylation factor GTPase-activating protein AGD5 (AGD5)

Code CSB-YP880782DOA
MSDS
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Source Yeast
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Code CSB-EP880782DOA
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Source E.coli
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Code CSB-EP880782DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP880782DOA
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Source Baculovirus
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Code CSB-MP880782DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGD5
Uniprot No.
Alternative Names
AGD5; MTV4; NEV; ZIG3; ZIGA3; At5g54310; MDK4.13ADP-ribosylation factor GTPase-activating protein AGD5; ARF GAP AGD5; Protein ARF-GAP DOMAIN 5; AtAGD5; Protein MODIFIED TRANSPORT TO THE VACUOLE 4; Protein NEVERSHED; Protein ZIGA3
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-483
Target Protein Sequence
MNEKANVSKE LNARHRKILE GLLKHPENRE CADCKTKGPR WASVNLGIFI CMQCSGIHRS LGVHISKVRS ATLDTWLPEQ VAFIQSMGND KANSYWEAEL PPNYDRVGIE NFIRAKYEEK RWVSRGEKAR SPPRVEQERR KSVERSGPGY EHGHSSSPVN LFEERKTIPA SRTRNNVAAT RINLPVPPQG PSQVIKPQQK MESAATPVER EKQAVNVAPA SDPPKVDFAT DLFNMLSMDD STTNTSEATP GDTPADDNSW AGFQSAGSGQ TAEKIVTAKP AESSSPPASS SDFEDLFKDT PNLTTQQAPK DVKGDIMSLF EKTNIVSPFA MHQQQVAMLA QQQALYMAAA KAAGGTPNGV NQQAIANALN VASANWSNPG GYQIPGMTNP VGGQADLQKL MQNMNMNANM NTRPAQPQEN TLQYPSSSFY TMGQANQVNG MTPNSTGKPQ SSSATQPTST TPSSQSGKDF DFSSLMDGMF TKH
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
GTPase-activating protein (GAP) for ADP ribosylation factor (ARF). Mediates clathrin-dependent trafficking of vacuolar cargo from the trans-Golgi network (TGN). Promotes plant growth. Involved in the regulation of membrane trafficking and cell separation during floral organ shedding and abscission. Prevents abscission zone (AZ) cells enlargement. Exhibits ARF-GTPase activity toward ARF1 at TGN.
Gene References into Functions
  1. IDA and NEV function during both the cell-wall loosening and the cell separation stages of the abscission process. In addition, NEV acts to inhibit premature cell expansion. PMID: 23963677
  2. AGD5, a a GTPase-activating protein, is distributed on a sub-population of the trans-Golgi network, where it co-localises with ARF1. PMID: 21105926
  3. NEV ARF-GAP activity at the trans-Golgi network and distinct endosomal compartments is required for the proper trafficking of cargo molecules required for cell separation. [NEV] PMID: 19429787
Subcellular Location
Golgi apparatus, trans-Golgi network. Endosome. Cytoplasmic vesicle, clathrin-coated vesicle.
Tissue Specificity
Expressed in inflorescence stems, abscission zones, stigmas, roots, roots meristems, embryos, and floral and leaf vasculatures.
Database Links

KEGG: ath:AT5G54310

STRING: 3702.AT5G54310.1

UniGene: At.20358

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