Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGO10 Polyclonal antibody

Uniprot No.

Q9XGW1

Target Names

AGO10

Alternative Names

AGO10 antibody; PNH antibody; ZLL antibody; At5g43810 antibody; MQD19.17Protein argonaute 10 antibody; Protein PINHEAD antibody; Protein ZWILLE antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AGO10 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Required for reliable formation of primary and axillary shoot apical meristems. Specifies leaf adaxial identity by repressing the miR165 and miR166 microRNAs in the embryonic shoot apex, in the shoot apical meristem (SAM) and leaf. Represses the microRNA miR398 which targets CCS1 chaperone mRNAs for translational inhibition. Acts as a negative regulator of AGO1 protein level. Like AGO1, is required for stem cell function and organ polarity. Unlike AGO1, is not subjected to small RNA-mediated repression itself. Essential for multiple processes in development. Coregulates, with GATA18/HAN, the shoot apical meristem (SAM) organization.

Gene References into Functions

the shoot regeneration inhibition determined by AGO10 functions via the repression of miR165/166. PMID: 29016889
The regulatory mechanism of AGO10 by which shoot apical meristem size is controlled under stress conditions.[LBD12-1] PMID: 27895202
data suggest that boundary-expressing HAN communicates with the meristem through the PNH, regulates floral organ development via JAG and BOP2, and maintains boundary morphology through CKX3 during flower development in Arabidopsis PMID: 26390296
Our results indicate that maintenance of shoot meristem stem cells by AGO10 involves negative regulation of auxin signaling and, via REV-mediated downregulation of ARF2 expression, auxin response. PMID: 26358077
AGO1, AGO2 and AGO10 promoted anti-TuMV defense in a modular way in various organs, with AGO2 providing a prominent antiviral role in leaves. AGO5, AGO7 and AGO10 had minor effects in leaves. PMID: 25806948
AGO10 quenches miR165/166 that move into AGO10-expressing niches and promotes shoot apical meristem maintenance. PMID: 25801022
AGO10 specifies leaf adaxial identity by repressing miR165/166. PMID: 19054365
ubiquitously expressed ARGONAUTE1 (AGO1) gene and its homolog AGO10, which exhibits highly restricted spatial expression patterns, are required to maintain the correct temporal program of floral stem cells PMID: 21483759
Using an unbiased biochemical approach, we show that AGO10 specifically binds to miR166/165. This association is determined by distinct structural features of the miR166/165 duplex. PMID: 21496644
proposed that ZWILLE buffers meristem stability non-cell-autonomously by ensuring the critical number of apical cells required for proper meristem function PMID: 14564520
Analysis of ago1 mutants with partially compromised AGO1 activity revealed that loss of ZLL function re-establishes both siRNA and miRNA pathways for a subset of AGO1 target genes. PMID: 19763164

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Subcellular Location

Cytoplasm.

Protein Families

Argonaute family, Ago subfamily

Tissue Specificity

Expressed in roots, stems, leaves, developing embryo, siliques, inflorescences, provascular tissue, shoot apical meristem (SAM) and adaxial (upper) sides of lateral organ primordia. Observed in the floral meristem, the adaxial side of sepal primordia, and

Database Links

KEGG: ath:AT5G43810

STRING: 3702.AT5G43810.1

UniGene: At.11438

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AGO10 Antibody

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