Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGO4 Polyclonal antibody

Uniprot No.

Q9ZVD5

Target Names

AGO4

Alternative Names

Protein argonaute 4 (Protein OVEREXPRESSOR OF CATIONIC PEROXIDASE 11) AGO4 OCP11 At2g27040 T20P8.9

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana AGO4 protein (101-360aa)

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

>95%, Protein G purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Recommended Dilution

Application	Recommended Dilution
WB	1:500-1:5000

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Usage

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

Target Background

Function

Together with RDM3, required for transcriptional gene silencing (TGS) by DNA methylation and repressive histone modifications (H3K9me2) of several chromatin loci. Component of the RISC complex that associate with the small interfering RNA (siRNA) pathway involved in direct cytosine methylation at endogenous DNA repeats. Forms a AGO4/NRPE1/siRNA complex in cajal body, facilitating its function in RNA-directed gene silencing of target loci. Required for CpNpG and asymmetric DNA methylation as well as histone H3 'Lys-9' methylation (H3K9me) at SUP and SN1 loci. May be not required for CpG methylation. Required for the production and maintenance of retrotransposon SN1 and Copia and ribosomal 5S 25 nucleotide siRNAs specialized in gene silencing at chromatin level. Involved in de novo methylation of FWA gene and required for the maintenance of RNA-directed DNA methylation (RdDM) triggered by inverted repeat transgenes. Interacts with miRNA miR390 and miR172, targeting respectively TAS3 and AP2 mRNAs, and mediates cleavage of miRNA targets. Associates mainly with small RNAs of 24 nucleotide in length and preferentially recruits small RNAs with a 5' terminal adenosine. Targeted by the turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO4 function in RNA silencing. Required for resistance to the bacterial pathogen P.syringae. Works independently of the RdDM pathway in mediating resistance to P.syringae. RdDM is involved in viral genome methylation as an epigenetic defense against geminiviruses.

Gene References into Functions

DDB2 forms a complex with ROS1 and AGO4 and that they act at the ROS1 locus to modulate levels of DNA methylation and therefore ROS1 expression. DDB2 acts as a critical regulator of ROS1-mediated active DNA demethylation. PMID: 29078035
These results indicate an important role for AGO4 in targeting plant RNA viruses as well as demonstrating novel mechanisms of regulation of and by AGO4, independent of its canonical role in regulating gene expression by RNA-dependent DNA methylation (RdDM). PMID: 27762650
Data suggest that AGO4-dependent siRNAs are secondary siRNAs dependent on the prior activity of the RdDM pathway at certain loci. PMID: 28002617
demonstrate that Pol V lncRNAs or the act of their transcription are required to lock Pol V holoenzyme into a stable DNA-bound state that allows AGO4 recruitment via redundant glycine-tryptophan/tryptophan-glycine AGO hook motifs present on both Pol V and its associated factor, SPT5L. PMID: 27986858
Findings reported here highlight an involvement of AGO4 during meiosis by ensuring accurate chromosome segregation at anaphase I. PMID: 27466226
Members of the AGO4 clade cooperatively participate in preventing the abnormal specification of multiple premeiotic gametophytic precursors during early ovule development. PMID: 27591749
Our results indicate that AtMBD6 is involved in RNA-mediated gene silencing and it binds to RNA binding proteins like AtRPS2C, AtAGO4 and AtNTF2. AtMBD6 also interacts with histone deacetylase AtHDA6 that might have a role in chromatin condensation at the targets of RdDM PMID: 28229965
These findings suggest that the targets of AGO4-directed RNA-directed DNA methylation are regulatory units responsible for controlling gene expression under specific environmental conditions. PMID: 23013441
AGO4 and AGO6 mainly act sequentially in mediating small RNA-directed DNA methylation. PMID: 25527293
Trans-acting small interfering RNAs are associated with AGO4 proteins to direct DNA methylation. PMID: 22846193
selective nuclear import of mature AGO4/siRNA complexes is a key regulatory point prior to the effector stage of RdDM. PMID: 22608924
DNA-dependent RNA polymerase IV-dependent smRNAs were mainly 24 nt and associated with AGO4. PMID: 21457371
AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent Pol V transcripts. [AGO4] [ARGONAUTE4] PMID: 19377477
Our results suggest a function for Cajal bodies as a center for the assembly of an AGO4/NRPD1b/siRNA complex, facilitating its function in RNA-directed gene silencing at target loci. PMID: 16839879
AGO4 binds to small RNAs including small interfering RNAs (siRNAs) originating from transposable and repetitive elements, and cleaves target RNA transcripts PMID: 16998468
AGO4 was partially compromised in the reception of silencing. PMID: 17785412
OCP11 was cloned and shown that it encodes ARGONAUTE4 (AGO4), a component of the pathway that mediates the transcriptional gene silencing associated with small interfering RNAs that direct DNA methylation at specific loci. PMID: 17993621
two classes of AGO4 bodies are structurally independent--disruption of one class does not affect the other--suggesting a dynamic regulation of AGO4 within two distinct nuclear compartments PMID: 18266474
SPT5-like emerged in plants as a facultative RNA polymerase elongation factor. PMID: 19343051
our data suggest that AGO4 is guided to target loci through base-pairing of associated siRNAs with nascent RNA Pol V transcripts. PMID: 19377477
The dual interaction of an effector protein with AGO and small RNA target transcripts may be a general feature of RNA-silencing effector complexes PMID: 19410546

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Subcellular Location

Nucleus, nucleolus. Nucleus, nucleoplasm. Nucleus, Cajal body.

Protein Families

Argonaute family, Ago subfamily

Tissue Specificity

Expressed in embryos, mature leaves, vascular tissue of the sepals, stamens and stigma, at the tip of the style and siliques.

Database Links

KEGG: ath:AT2G27040

STRING: 3702.AT2G27040.1

UniGene: At.13165

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Code	CSB-PA935743XA01DOA
Size	US$299
Image	Western Blot Positive WB detected in: recombinant protein All lanes: AGO4 Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 33 kDa Observed band size: 33 kDa
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AGO4 Antibody

Product Details

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Target Background