AGO9 Antibody

Code CSB-PA801039XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AGO9 Polyclonal antibody
Uniprot No.
Target Names
AGO9
Alternative Names
AGO9 antibody; At5g21150 antibody; T10F18.180Protein argonaute 9 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AGO9 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates preferentially with small RNAs of 24 nucleotide in length with a 5' terminal adenosine. Interacts with 24 nucleotide sRNAs derived from transposable elements (TEs). Required to silence pericentrometric-located TEs in female gametes and their accessory cells. Necessary to inactivate a significant proportion of long terminal repeat retrotransposons (LTRs) in the ovule. Required to specify cell fate in ovule. Involved in the control of female gamete formation by restricting the specification of gametophyte precursors in a dosage-dependent, non-cell-autonomous manner. Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO9 function in RNA silencing.
Gene References into Functions
  1. AGO9 can bind to 24 retrotransposons and its predominant transposable element targets are in the pericentromeric regions of all 5 chromosomes. PMID: 21057207
  2. Data show that AGO9 gene (At5G21150) was preferentially expressed in anthers versus meiocytes (M/A = 78.24/172.37), which is consistent with the discovery of a postmeiotic function. PMID: 21167045
  3. Arabidopsis protein ARGONAUTE 9 (AGO9) controls female gamete formation by restricting the specification of gametophyte precursors in a dosage-dependent, non-cell-autonomous manner. [ARGONAUTE 9] [AGO9] PMID: 20208518
Protein Families
Argonaute family, Ago subfamily
Tissue Specificity
Expressed in embryonic shoot apex region, pollen and developing ovules.
Database Links

KEGG: ath:AT5G21150

STRING: 3702.AT5G21150.1

UniGene: At.31104

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