Recombinant Arabidopsis thaliana Protein argonaute 9 (AGO9), partial

Code CSB-YP801039DOA
MSDS
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Source Yeast
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Code CSB-EP801039DOA
MSDS
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Source E.coli
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Code CSB-EP801039DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP801039DOA
MSDS
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Source Baculovirus
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Code CSB-MP801039DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AGO9
Uniprot No.
Alternative Names
AGO9; At5g21150; T10F18.180Protein argonaute 9
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Involved in RNA-mediated post-transcriptional gene silencing (PTGS). Main component of the RNA-induced silencing complex (RISC) that binds to a short guide RNA such as a microRNA (miRNA) or small interfering RNA (siRNA). RISC uses the mature miRNA or siRNA as a guide for slicer-directed cleavage of homologous mRNAs to repress gene expression. Associates preferentially with small RNAs of 24 nucleotide in length with a 5' terminal adenosine. Interacts with 24 nucleotide sRNAs derived from transposable elements (TEs). Required to silence pericentrometric-located TEs in female gametes and their accessory cells. Necessary to inactivate a significant proportion of long terminal repeat retrotransposons (LTRs) in the ovule. Required to specify cell fate in ovule. Involved in the control of female gamete formation by restricting the specification of gametophyte precursors in a dosage-dependent, non-cell-autonomous manner. Targeted by turnip yellows virus (TuYV) protein P0 (via F-box-like domain) for probable proteasome degradation and thereby inactivating AGO9 function in RNA silencing.
Gene References into Functions
  1. AGO9 can bind to 24 retrotransposons and its predominant transposable element targets are in the pericentromeric regions of all 5 chromosomes. PMID: 21057207
  2. Data show that AGO9 gene (At5G21150) was preferentially expressed in anthers versus meiocytes (M/A = 78.24/172.37), which is consistent with the discovery of a postmeiotic function. PMID: 21167045
  3. Arabidopsis protein ARGONAUTE 9 (AGO9) controls female gamete formation by restricting the specification of gametophyte precursors in a dosage-dependent, non-cell-autonomous manner. [ARGONAUTE 9] [AGO9] PMID: 20208518
Protein Families
Argonaute family, Ago subfamily
Tissue Specificity
Expressed in embryonic shoot apex region, pollen and developing ovules.
Database Links

KEGG: ath:AT5G21150

STRING: 3702.AT5G21150.1

UniGene: At.31104

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