ALMT12 Antibody

Code CSB-PA526193XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ALMT12 Polyclonal antibody
Uniprot No.
Target Names
ALMT12
Alternative Names
ALMT12; QUAC1; At4g17970; T6K21.150; Aluminum-activated malate transporter 12; AtALMT12; Quick anion channel 1
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ALMT12 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Malate-sensitive anion transporter permeable to chloride, nitrate, sulfate and malate. Involved in dark-, CO(2)-, abscisic acid- and water-deficient-induced stomatal closure. Belongs to the R-type anion channels.
Gene References into Functions
  1. ALMT12 role in drought induced stomatal closure PMID: 28446637
  2. QUAC1, an R-type anion channel responsible for the release of malate from guard cells, is essential for efficient stomatal closure and plays a role in mesophyll conductance. PMID: 26542441
  3. In the presence of abscissic acid, OST1 is able to phosphorylate/activate the plant anion channels QUAC1 and SLAC1, leading to stomatal closure.[QUAC1] PMID: 23452338
  4. The cytosolic C-terminus is involved in the voltage-dependent gating mechanism of QUAC1. PMID: 23314055
  5. Activity of heterologously expressed AtALMT12 depends on extracellular malate. Thereby this key metabolite and osmolite of guard cells shifts the threshold for voltage activation of AtALMT12 towards more hyperpolarized potentials. PMID: 20626656
  6. AtALMT12 is a novel class of anion transporter involved in stomatal closure. PMID: 20154005

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Subcellular Location
Cell membrane; Multi-pass membrane protein. Note=PubMed:20626656 indicates also a not confirmed endomembrane localization.
Protein Families
Aromatic acid exporter (TC 2.A.85) family
Tissue Specificity
Expressed in roots, stems, leaves, flowers and pollen. Mainly detected in the roots vascular stele and in the leaves guard cells.
Database Links

KEGG: ath:AT4G17970

STRING: 3702.AT4G17970.1

UniGene: At.2053

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