ani-1 Antibody

Code CSB-PA893178XA01CXY
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Product Details

Full Product Name
Rabbit anti-Caenorhabditis elegans ani-1 Polyclonal antibody
Uniprot No.
Target Names
ani-1
Alternative Names
ani-1 antibody; Y49E10.19Anillin-like protein 1 antibody
Raised in
Rabbit
Species Reactivity
Caenorhabditis elegans
Immunogen
Recombinant Caenorhabditis elegans ani-1 protein
Immunogen Species
Caenorhabditis elegans
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for contractile events in embryos that occur prior to mitosis, such as cortical ruffling and pseudocleavage. Promotes membrane ruffling by organizing cortical patches of septins and myosin II. Not generally required for cytokinesis in mitotic cells. Required for the asymmetric cleavage events that extrude the two polar bodies during oocyte meiosis. Not required for meiotic contractile ring assembly, initiation or closure but is required for the transformation of the contractile ring from a disk above the spindle to a tube around the spindle midzone. Promotes astral microtubule-directed cortical myosin polarization and cleavage furrow ingression. Regulates neuroblast cytokinesis during mid- to late-embryogenesis and is required for ventral enclosure.
Gene References into Functions
  1. ANI-1 and ANI-2 contribute to embryonic viability by performing distinct functions in the early embryo and gonad, respectively [ANI-1 & ANI-2] PMID: 15930113
  2. active form of RhoG/MIG-2 directly binds to Anillin and recruits it to the leading edge of Q neuroblasts PMID: 25843030
  3. Caenorhabditis elegans anillin (ani-1) regulates neuroblast cytokinesis and epidermal morphogenesis during embryonic development. PMID: 24016757
  4. Anillin-1 promotes astral microtubule-directed cortical myosin polarization PMID: 21737681
  5. Anillin is dispensable for contractile ring assembly, initiation, and closure but is required for the meiotic contractile ring to transform from a disc into a tube. PMID: 21055941
  6. Asymmetric ingression requires Anillin and the septins, which promote the coalescence of components on one side of the contractile ring, but is insensitive to a 10-fold reduction in myosin II levels. PMID: 17488632

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Subcellular Location
Cytoplasm, cell cortex. Cytoplasm, cytoskeleton. Cytoplasm, cytoskeleton, spindle. Midbody. Cleavage furrow.
Tissue Specificity
Strongly expressed in dividing neuroblasts under the ventral epidermal cells during ventral enclosure.
Database Links
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