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Recombinant Anillin-like protein 1 (ani-1), partial

Recombinant Anillin-like protein 1 (ani-1), partial

Code	CSB-YP893178CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Yeast
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Code	CSB-EP893178CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
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Code	CSB-EP893178CXY-B
MSDS	MSDS Datasheet
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP893178CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP893178CXY
MSDS	MSDS Datasheet
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ani-1

Uniprot No.

Q9XTT4

Alternative Names

ani-1; Y49E10.19Anillin-like protein 1

Species

Caenorhabditis elegans

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Required for contractile events in embryos that occur prior to mitosis, such as cortical ruffling and pseudocleavage. Promotes membrane ruffling by organizing cortical patches of septins and myosin II. Not generally required for cytokinesis in mitotic cells. Required for the asymmetric cleavage events that extrude the two polar bodies during oocyte meiosis. Not required for meiotic contractile ring assembly, initiation or closure but is required for the transformation of the contractile ring from a disk above the spindle to a tube around the spindle midzone. Promotes astral microtubule-directed cortical myosin polarization and cleavage furrow ingression. Regulates neuroblast cytokinesis during mid- to late-embryogenesis and is required for ventral enclosure.

Gene References into Functions

ANI-1 and ANI-2 contribute to embryonic viability by performing distinct functions in the early embryo and gonad, respectively [ANI-1 & ANI-2] PMID: 15930113
active form of RhoG/MIG-2 directly binds to Anillin and recruits it to the leading edge of Q neuroblasts PMID: 25843030
Caenorhabditis elegans anillin (ani-1) regulates neuroblast cytokinesis and epidermal morphogenesis during embryonic development. PMID: 24016757
Anillin-1 promotes astral microtubule-directed cortical myosin polarization PMID: 21737681
Anillin is dispensable for contractile ring assembly, initiation, and closure but is required for the meiotic contractile ring to transform from a disc into a tube. PMID: 21055941
Asymmetric ingression requires Anillin and the septins, which promote the coalescence of components on one side of the contractile ring, but is insensitive to a 10-fold reduction in myosin II levels. PMID: 17488632