ANT Antibody

Code CSB-PA657128XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ANT Polyclonal antibody
Uniprot No.
Target Names
ANT
Alternative Names
ANT antibody; CKC1 antibody; DRG antibody; OVM antibody; At4g37750 antibody; T28I19.30AP2-like ethylene-responsive transcription factor ANT antibody; Complementing a protein kinase C mutant protein 1 antibody; Protein AINTEGUMENTA antibody; Protein DRAGON antibody; Protein OVULE MUTANT antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ANT protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CAC[AG]N[AT]TNCCNANG-3'. Required for the initiation and growth of ovules integumenta, and for the development of female gametophyte. Plays a critical role in the development of gynoecium marginal tissues (e.g. stigma, style and septa), and in the fusion of carpels and of medial ridges leading to ovule primordia. Also involved in organs initiation and development, including floral organs. Maintains the meristematic competence of cells and consequently sustains expression of cell cycle regulators during organogenesis, thus controlling the final size of each organ by controlling their cell number. Regulates INO autoinduction and expression pattern. As ANT promotes petal cell identity and mediates down-regulation of AG in flower whorl 2, it functions as a class A homeotic gene.
Gene References into Functions
  1. ANT regulates biological pathways that are critical for cell wall remodeling and defense. PMID: 27208279
  2. ANT and AIL6 directly induce LFY expression in response to auxin to promote the onset of flower formation. PMID: 26537561
  3. ANT is a novel regulator of salt stress and that ANT binds to the SCABP8 promoter, mediating salt tolerance. PMID: 26054800
  4. Overexpression of AtANT in Antirrhinum caused increased flower size via increased petal limb width and tube length. PMID: 24985495
  5. these three transcription factors LFY, ANT and AIL6/PLT3 are redundantly required for flower primordium initiation. PMID: 23375585
  6. ANT, AIL6 and AIL7 are expressed in distinct domains within the vegetative shoot apical meristem. Triple mutant shoot apical meristems exhibit morphological and cell division defects. PMID: 22380923
  7. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). PMID: 22031826
  8. ANT is nuclear localized and that the sequence KKKR (amino acids 252-255) is required for nuclear localization of the protein. PMID: 16523347
  9. ANT acts in combination with the YABBY gene FILAMENTOUS FLOWER (FIL) to promote organ polarity by up-regulating the expression of the adaxial-specifying HD-ZIP gene PHABULOSA. PMID: 16714408
  10. SEU and ANT are part of a complex and robust molecular system that coordinates patterning cues and cellular proliferation along the three positional axes of the developing gynoecium. PMID: 18184731
  11. ANT and AIL6 are important regulators of floral growth and patterning and that they may act downstream of auxin in these processes. PMID: 19542297

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, AP2 subfamily
Tissue Specificity
Mostly expressed in developing flowers. Also present in mature flowers, siliques and seedlings, but not in mature roots, leaves and stems. Expressed in ovules and in vegetative and floral primordia.
Database Links

KEGG: ath:AT4G37750

STRING: 3702.AT4G37750.1

UniGene: At.1556

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