Recombinant Arabidopsis thaliana AP2-like ethylene-responsive transcription factor ANT (ANT), partial

Code CSB-YP657128DOA
MSDS
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Source Yeast
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Code CSB-EP657128DOA
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Source E.coli
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Code CSB-EP657128DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP657128DOA
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Source Baculovirus
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Code CSB-MP657128DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ANT
Uniprot No.
Alternative Names
ANT; CKC1; DRG; OVM; At4g37750; T28I19.30AP2-like ethylene-responsive transcription factor ANT; Complementing a protein kinase C mutant protein 1; Protein AINTEGUMENTA; Protein DRAGON; Protein OVULE MUTANT
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcription activator that recognizes and binds to the DNA consensus sequence 5'-CAC[AG]N[AT]TNCCNANG-3'. Required for the initiation and growth of ovules integumenta, and for the development of female gametophyte. Plays a critical role in the development of gynoecium marginal tissues (e.g. stigma, style and septa), and in the fusion of carpels and of medial ridges leading to ovule primordia. Also involved in organs initiation and development, including floral organs. Maintains the meristematic competence of cells and consequently sustains expression of cell cycle regulators during organogenesis, thus controlling the final size of each organ by controlling their cell number. Regulates INO autoinduction and expression pattern. As ANT promotes petal cell identity and mediates down-regulation of AG in flower whorl 2, it functions as a class A homeotic gene.
Gene References into Functions
  1. ANT regulates biological pathways that are critical for cell wall remodeling and defense. PMID: 27208279
  2. ANT and AIL6 directly induce LFY expression in response to auxin to promote the onset of flower formation. PMID: 26537561
  3. ANT is a novel regulator of salt stress and that ANT binds to the SCABP8 promoter, mediating salt tolerance. PMID: 26054800
  4. Overexpression of AtANT in Antirrhinum caused increased flower size via increased petal limb width and tube length. PMID: 24985495
  5. these three transcription factors LFY, ANT and AIL6/PLT3 are redundantly required for flower primordium initiation. PMID: 23375585
  6. ANT, AIL6 and AIL7 are expressed in distinct domains within the vegetative shoot apical meristem. Triple mutant shoot apical meristems exhibit morphological and cell division defects. PMID: 22380923
  7. SEUSS (SEU) and AINTEGUMENTA (ANT) encode transcriptional regulators that are critical for the proper formation of ovules from the carpel margin meristem (CMM). PMID: 22031826
  8. ANT is nuclear localized and that the sequence KKKR (amino acids 252-255) is required for nuclear localization of the protein. PMID: 16523347
  9. ANT acts in combination with the YABBY gene FILAMENTOUS FLOWER (FIL) to promote organ polarity by up-regulating the expression of the adaxial-specifying HD-ZIP gene PHABULOSA. PMID: 16714408
  10. SEU and ANT are part of a complex and robust molecular system that coordinates patterning cues and cellular proliferation along the three positional axes of the developing gynoecium. PMID: 18184731
  11. ANT and AIL6 are important regulators of floral growth and patterning and that they may act downstream of auxin in these processes. PMID: 19542297

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, AP2 subfamily
Tissue Specificity
Mostly expressed in developing flowers. Also present in mature flowers, siliques and seedlings, but not in mature roots, leaves and stems. Expressed in ovules and in vegetative and floral primordia.
Database Links

KEGG: ath:AT4G37750

STRING: 3702.AT4G37750.1

UniGene: At.1556

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