AP2 Antibody

Code CSB-PA342522XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) AP2 Polyclonal antibody
Uniprot No.
Target Names
AP2
Alternative Names
AP2 antibody; At4g36920 antibody; AP22.49 antibody; C7A10.440Floral homeotic protein APETALA 2 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana AP2 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Probable transcriptional activator that promotes early floral meristem identity. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower, by spatially controlling the expression domains of multiple floral organ identity genes. Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS. Directly represses AGAMOUS by recruiting the transcriptional corepressor TOPLESS and the histone deacetylase HDA19. It is also required during seed development.
Gene References into Functions
  1. AtFLO2 is strongly involved in regulation of translocation and transport of assimilates, and contributes greatly to quality control of the various processes involving substance supply or transfer, such as photoassimilation, leaf enlargement, yield of seeds in a silique and accumulation of seed storage compounds. PMID: 28158741
  2. Data show that FRUITFULL (FUL), a MADS-box gene involved in flowering and fruit development, has a key role in promoting meristem arrest, directly and negatively regulates APETALA2 expression in the shoot apical meristem. PMID: 29422669
  3. APETALA2 Gene Promoter Is Bidirectional and Functions as a Pollen- and Ovule-Specific Promoter in the Reverse Orientation PMID: 28130768
  4. upregulation of LINC-AP2 is negatively correlated with AP2 gene expression with Turnip crinkle virus infection in Arabidopsis. PMID: 27473526
  5. findings show that AP2 represses its target genes by physically recruiting the co-repressor TOPLESS and the histone deacetylase HDA19 PMID: 23034631
  6. APETALA2 recognizes and acts through an AT-rich sequence element to promote sepal and petal identities and restrict expression of AGAMOUS in whorls 1 and 2. PMID: 22513376
  7. AP2 acts to prevent replum overgrowth in developing fruit by negatively regulating BP and RPL, two genes that normally act to promote replum formation. PMID: 22031547
  8. AP2 influences development of the zygotic embryo and endosperm to repress seed size. PMID: 20033449
  9. The work demonstrates a novel role for BPMs as potential regulators that affect transcriptional activities of ERF/AP2 proteins. PMID: 19843165
  10. Control of seed mass and seed yield by the floral homeotic gene APETALA2. PMID: 15708974
  11. a role for AP2 in controlling seed mass. PMID: 15708976
  12. Genetic analysis shows that termination of the primary shoot meristem in l28 mutants requires an active CLV signaling pathway, indicating that AP2 functions in stem cell maintenance by modifying the WUS-CLV3 feedback loop. PMID: 16387832
  13. The expression of AP2 under the control of miR172 in N. benthiana is reported. PMID: 16897492
  14. miR172, a microRNA, serves as a negative regulator of AP2. PMID: 17573799

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, AP2 subfamily
Tissue Specificity
Sepals, petals, stamens, carpels, developing ovules, inflorescence stem, leaf and stem.
Database Links

KEGG: ath:AT4G36920

STRING: 3702.AT4G36920.1

UniGene: At.4638

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