Recombinant Arabidopsis thaliana Floral homeotic protein APETALA 2 (AP2)

Code CSB-YP342522DOA
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Source Yeast
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Code CSB-EP342522DOA
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Source E.coli
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Code CSB-EP342522DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP342522DOA
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Source Baculovirus
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Code CSB-MP342522DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
AP2
Uniprot No.
Alternative Names
AP2; At4g36920; AP22.49; C7A10.440Floral homeotic protein APETALA 2
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-432
Target Protein Sequence
MWDLNDAPHQ TQREEESEEF CYSSPSKRVG SFSNSSSSAV VIEDGSDDDE LNRVRPNNPL VTHQFFPEMD SNGGGVASGF PRAHWFGVKF CQSDLATGSS AGKATNVAAA VVEPAQPLKK SRRGPRSRSS QYRGVTFYRR TGRWESHIWD CGKQVYLGGF DTAHAAARAY DRAAIKFRGV EADINFNIDD YDDDLKQMTN LTKEEFVHVL RRQSTGFPRG SSKYRGVTLH KCGRWEARMG QFLGKKYVYL GLFDTEVEAA RAYDKAAIKC NGKDAVTNFD PSIYDEELNA ESSGNPTTPQ DHNLDLSLGN SANSKHKSQD MRLRMNQQQQ DSLHSNEVLG LGQTGMLNHT PNSNHQFPGS SNIGSGGGFS LFPAAENHRF DGRASTNQVL TNAAASSGFS PHHHNQIFNS TSTPHQNWLQ TNGFQPPLMR PS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Probable transcriptional activator that promotes early floral meristem identity. Is required subsequently for the transition of an inflorescence meristem into a floral meristem. Plays a central role in the specification of floral identity, particularly for the normal development of sepals and petals in the wild-type flower, by spatially controlling the expression domains of multiple floral organ identity genes. Acts as A class cadastral protein by repressing the C class floral homeotic gene AGAMOUS in association with other repressors like LEUNIG and SEUSS. Directly represses AGAMOUS by recruiting the transcriptional corepressor TOPLESS and the histone deacetylase HDA19. It is also required during seed development.
Gene References into Functions
  1. AtFLO2 is strongly involved in regulation of translocation and transport of assimilates, and contributes greatly to quality control of the various processes involving substance supply or transfer, such as photoassimilation, leaf enlargement, yield of seeds in a silique and accumulation of seed storage compounds. PMID: 28158741
  2. Data show that FRUITFULL (FUL), a MADS-box gene involved in flowering and fruit development, has a key role in promoting meristem arrest, directly and negatively regulates APETALA2 expression in the shoot apical meristem. PMID: 29422669
  3. APETALA2 Gene Promoter Is Bidirectional and Functions as a Pollen- and Ovule-Specific Promoter in the Reverse Orientation PMID: 28130768
  4. upregulation of LINC-AP2 is negatively correlated with AP2 gene expression with Turnip crinkle virus infection in Arabidopsis. PMID: 27473526
  5. findings show that AP2 represses its target genes by physically recruiting the co-repressor TOPLESS and the histone deacetylase HDA19 PMID: 23034631
  6. APETALA2 recognizes and acts through an AT-rich sequence element to promote sepal and petal identities and restrict expression of AGAMOUS in whorls 1 and 2. PMID: 22513376
  7. AP2 acts to prevent replum overgrowth in developing fruit by negatively regulating BP and RPL, two genes that normally act to promote replum formation. PMID: 22031547
  8. AP2 influences development of the zygotic embryo and endosperm to repress seed size. PMID: 20033449
  9. The work demonstrates a novel role for BPMs as potential regulators that affect transcriptional activities of ERF/AP2 proteins. PMID: 19843165
  10. Control of seed mass and seed yield by the floral homeotic gene APETALA2. PMID: 15708974
  11. a role for AP2 in controlling seed mass. PMID: 15708976
  12. Genetic analysis shows that termination of the primary shoot meristem in l28 mutants requires an active CLV signaling pathway, indicating that AP2 functions in stem cell maintenance by modifying the WUS-CLV3 feedback loop. PMID: 16387832
  13. The expression of AP2 under the control of miR172 in N. benthiana is reported. PMID: 16897492
  14. miR172, a microRNA, serves as a negative regulator of AP2. PMID: 17573799

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Subcellular Location
Nucleus.
Protein Families
AP2/ERF transcription factor family, AP2 subfamily
Tissue Specificity
Sepals, petals, stamens, carpels, developing ovules, inflorescence stem, leaf and stem.
Database Links

KEGG: ath:AT4G36920

STRING: 3702.AT4G36920.1

UniGene: At.4638

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