APRR5 Antibody

Code CSB-PA753819XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) APRR5 Polyclonal antibody
Uniprot No.
Target Names
APRR5
Alternative Names
APRR5 antibody; At5g24470 antibody; T31K7.5Two-component response regulator-like APRR5 antibody; Pseudo-response regulator 5 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana APRR5 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional repressor of CCA1 and LHY, thereby controlling photoperiodic flowering response. Involved in the positive and negative feedback loops of the circadian clock. With RVE8, forms a negative feedback loop of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Proteolytic substrate of the E3 ubiquitin ligase SCF(ADO1) complex. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Negative regulator of shade avoidance response. Involved in the inhibition of leaf expansion in shade avoidance response.
Gene References into Functions
  1. Data report that COR27 and COR28 bind to the chromatin of TOC1 and PRR5 to repress their transcription, suggesting that their effects on rhythms are in part due to their regulation of TOC1 and PRR5 These data demonstrate that blue light and low temperature-regulated COR27 and COR28 regulate the circadian clock as well as freezing tolerance and flowering time. PMID: 27837007
  2. LNK1 is a transcriptional coactivator necessary for expression of the clock genes PRR5 and TOC1 through recruitment to their promoters via interaction with bona fide DNA binding proteins such as RVE4 and RVE8. PMID: 25012192
  3. Expression analyses of shade avoidance response (S.A.R)-related genes suggested that PRR5 functions as a repressor of S.A.R. Analyses of leaves under different light condition revealed that PRR5 is involved in the inhibition of leaf expansion in S.A.R. PMID: 23333981
  4. Transcriptional co-regulators PRR9, PRR7 and PRR5 inhibit morning loops LHY and CCA1 expression by binding to their promoters PMID: 24267177
  5. RVE8 promotes expression of the clock component PSEUDO-RESPONSE REGULATOR 5 (PRR5). RVE8 expression is in turn repressed by PRR5. PMID: 21483796
  6. PRR5 plays an antagonistic role(s) to the putative CCA1 clock component.[PRR5] [PSEUDO-RESPONSE REGULATOR 5] PMID: 15725674
  7. PRR5 seedlings showed a phenotype of hypersensitivity to red light during early photomorphogenesis PMID: 17284847
  8. These results show that ZTL targets PRR5 for degradation by 26S proteasomes in the circadian clock and in early photomorphogenesis. PMID: 17693530
  9. The linkages between TOC1 and PRR5 in the regulation of the biological clock of A. thaliana were studied. PMID: 18178585
  10. revealed the genetic linkages between GI and PRR5 in the control of free running circadian oscillation of gene expression, early photomorphogenesis and flowering time PMID: 18838788

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Subcellular Location
Nucleus.
Protein Families
ARR-like family
Database Links

KEGG: ath:AT5G24470

STRING: 3702.AT5G24470.1

UniGene: At.21641

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