Product Details

Purity

>85% (SDS-PAGE)

Target Names

APRR5

Uniprot No.

Q6LA42

Alternative Names

APRR5; At5g24470; T31K7.5Two-component response regulator-like APRR5; Pseudo-response regulator 5

Species

Arabidopsis thaliana (Mouse-ear cress)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose.

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Target Background

Function

Transcriptional repressor of CCA1 and LHY, thereby controlling photoperiodic flowering response. Involved in the positive and negative feedback loops of the circadian clock. With RVE8, forms a negative feedback loop of the circadian clock. Expression of several members of the ARR-like family is controlled by circadian rhythm. Proteolytic substrate of the E3 ubiquitin ligase SCF(ADO1) complex. APRR9, APRR7, and APRR5 coordinately act on the upstream region of the target genes to repress their expression from noon until midnight. The particular coordinated sequential expression of APRR9, APRR7, APRR5, APRR3 and APPR1 result to circadian waves that may be at the basis of the endogenous circadian clock. Negative regulator of shade avoidance response. Involved in the inhibition of leaf expansion in shade avoidance response.

Gene References into Functions

Data report that COR27 and COR28 bind to the chromatin of TOC1 and PRR5 to repress their transcription, suggesting that their effects on rhythms are in part due to their regulation of TOC1 and PRR5 These data demonstrate that blue light and low temperature-regulated COR27 and COR28 regulate the circadian clock as well as freezing tolerance and flowering time. PMID: 27837007
LNK1 is a transcriptional coactivator necessary for expression of the clock genes PRR5 and TOC1 through recruitment to their promoters via interaction with bona fide DNA binding proteins such as RVE4 and RVE8. PMID: 25012192
Expression analyses of shade avoidance response (S.A.R)-related genes suggested that PRR5 functions as a repressor of S.A.R. Analyses of leaves under different light condition revealed that PRR5 is involved in the inhibition of leaf expansion in S.A.R. PMID: 23333981
Transcriptional co-regulators PRR9, PRR7 and PRR5 inhibit morning loops LHY and CCA1 expression by binding to their promoters PMID: 24267177
RVE8 promotes expression of the clock component PSEUDO-RESPONSE REGULATOR 5 (PRR5). RVE8 expression is in turn repressed by PRR5. PMID: 21483796
PRR5 plays an antagonistic role(s) to the putative CCA1 clock component.[PRR5] [PSEUDO-RESPONSE REGULATOR 5] PMID: 15725674
PRR5 seedlings showed a phenotype of hypersensitivity to red light during early photomorphogenesis PMID: 17284847
These results show that ZTL targets PRR5 for degradation by 26S proteasomes in the circadian clock and in early photomorphogenesis. PMID: 17693530
The linkages between TOC1 and PRR5 in the regulation of the biological clock of A. thaliana were studied. PMID: 18178585
revealed the genetic linkages between GI and PRR5 in the control of free running circadian oscillation of gene expression, early photomorphogenesis and flowering time PMID: 18838788

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Subcellular Location

Nucleus.

Protein Families

ARR-like family

Database Links

KEGG: ath:AT5G24470

STRING: 3702.AT5G24470.1

UniGene: At.21641

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Recombinant Arabidopsis thaliana Two-component response regulator-like APRR5 (APRR5), partial

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