ari-1 Antibody

Code CSB-PA839104XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) ari-1 Polyclonal antibody
Uniprot No.
Target Names
ari-1
Alternative Names
ari-1 antibody; ari antibody; ari-1a antibody; CG5659E3 ubiquitin-protein ligase ariadne-1 antibody; EC 2.3.2.31 antibody; Protein ariadne-1 antibody; RING-type E3 ubiquitin transferase ariadne-1 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) ari-1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Atypical E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 Ubc10. Controls the subcellular localization and morphology of muscle nuclei (myonuclei) by regulating the protein levels and distribution of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex. Functions by mediating the monoubiquitination of the LINC complex subunit koi leading to its subsequent proteasomal degradation. Appears to function, at least partially redundantly, with the RBR E3 ligase family member park in nuclear localization and morphology. Likely to function in metamorphosis by regulating the proteins levels of EcR isoform A (ECR-A) and its heterodimeric partner usp, via the ubiquitination and subsequent degradation of ECR-A.
Gene References into Functions
  1. Variants in ARI-1 are associated with nuclear clustering and morphology defects in larval muscles and thus developing aortic aneurysms. PMID: 29689197
  2. Ariadne-1a binds specifically the isoform A of EcR and ubiquitylates it PMID: 21900267
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
RBR family, Ariadne subfamily
Tissue Specificity
Widely expressed, with prominent levels in the nervous system and female gonads.
Database Links

KEGG: dme:Dmel_CG5659

STRING: 7227.FBpp0297199

UniGene: Dm.6280

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