Recombinant Drosophila melanogaster Protein ariadne-1 (ari-1), partial

Code CSB-YP839104DLU
MSDS
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Source Yeast
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Code CSB-EP839104DLU
MSDS
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Source E.coli
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Code CSB-EP839104DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839104DLU
MSDS
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Source Baculovirus
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Code CSB-MP839104DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ari-1
Uniprot No.
Alternative Names
ari-1; ari; ari-1a; CG5659E3 ubiquitin-protein ligase ariadne-1; EC 2.3.2.31; Protein ariadne-1; RING-type E3 ubiquitin transferase ariadne-1
Species
Drosophila melanogaster (Fruit fly)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Atypical E3 ubiquitin-protein ligase, which catalyzes ubiquitination of target proteins together with ubiquitin-conjugating enzyme E2 Ubc10. Controls the subcellular localization and morphology of muscle nuclei (myonuclei) by regulating the protein levels and distribution of the LINC (LInker of Nucleoskeleton and Cytoskeleton) complex. Functions by mediating the monoubiquitination of the LINC complex subunit koi leading to its subsequent proteasomal degradation. Appears to function, at least partially redundantly, with the RBR E3 ligase family member park in nuclear localization and morphology. Likely to function in metamorphosis by regulating the proteins levels of EcR isoform A (ECR-A) and its heterodimeric partner usp, via the ubiquitination and subsequent degradation of ECR-A.
Gene References into Functions
  1. Variants in ARI-1 are associated with nuclear clustering and morphology defects in larval muscles and thus developing aortic aneurysms. PMID: 29689197
  2. Ariadne-1a binds specifically the isoform A of EcR and ubiquitylates it PMID: 21900267
Subcellular Location
Cytoplasm. Nucleus.
Protein Families
RBR family, Ariadne subfamily
Tissue Specificity
Widely expressed, with prominent levels in the nervous system and female gonads.
Database Links

KEGG: dme:Dmel_CG5659

STRING: 7227.FBpp0297199

UniGene: Dm.6280

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