ark1 Antibody

Code CSB-PA528879XA01SXV
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Product Details

Full Product Name
Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) ark1 Polyclonal antibody
Uniprot No.
Target Names
ark1
Alternative Names
ark1 antibody; sex1 antibody; SPCC320.13c antibody; SPCC330.16Serine/threonine-protein kinase ark1 antibody; EC 2.7.11.1 antibody; Aurora-related kinase 1 antibody
Raised in
Rabbit
Species Reactivity
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen
Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) ark1 protein
Immunogen Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required for the spindle checkpoint attachment response during spindle formation, kinetochore microtubule interactions and chromosome segregation during anaphase. Ark1 activity depends upon cut17 function and phosphorylation. Ark1 with bir1 is required for full-scale association with kinetochores and formation of a complex with mad3. Ark1 is also required for phosphorylation of histone H3 that accompanies chromosome condensation and condensin recruitment to mitotic chromatin. Ark1 with pic1 is required for the execution of cytokinesis.
Gene References into Functions
  1. A new role for Aurora B in telomere dispersion and disjunction during fission yeast mitosis. PMID: 25778919
  2. promotes cell division during recovery from TOR-mediated cell cycle arrest by driving spindle pole body recruitment of Polo PMID: 21965528
  3. genetic and cytological analyses suggest that the interplay of chiasmata and kinetochore geometry leads to correct silencing of Aurora B activity at centromeres, providing insight into how chiasmata regulate meiotic chromosome orientation. PMID: 21920317
  4. the Cnd2 subunit of condensin is the target subunit of Aurora-B-like kinase Ark1 and that Ark1-mediated phosphorylation of Cnd2 occurred throughout mitosis PMID: 21540296
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Associates with the elongating spindle during anaphase.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
Database Links
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Call us
301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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Address
7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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