Recombinant Schizosaccharomyces pombe Serine/threonine-protein kinase ark1 (ark1)

Code CSB-YP528879SXV
MSDS
Size Pls inquire
Source Yeast
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Code CSB-EP528879SXV
MSDS
Size Pls inquire
Source E.coli
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Code CSB-EP528879SXV-B
MSDS
Size Pls inquire
Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP528879SXV
MSDS
Size Pls inquire
Source Baculovirus
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Code CSB-MP528879SXV
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ark1
Uniprot No.
Alternative Names
ark1; sex1; SPCC320.13c; SPCC330.16Serine/threonine-protein kinase ark1; EC 2.7.11.1; Aurora-related kinase 1
Species
Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Expression Region
1-355
Target Protein Sequence
MSDSKLADSL NCLSVSTPST TANPGRQQLL RLAVSNQRQV NNVSLANGKE NKRTSNSKFN SSLRKIEEPI AGVPSSAGPQ WREFHIGMFE IGKPLGKGKF GRVYLAKEKK TGFIVALKTL HKSELVQSKI EKQVRREIEI QSNLRHKNIL RLYGHFHDEK RIYLILEFAG RGELYQHLRR AKRFSEEVAS KYIFQMANAL SYLHKKHVIH RDIKPENILL GIDGEIKLSD FGWSVHAPSN RRTTLCGTLD YLPPEMVEGK EHTEKVDLWS LGVLTYEFLV GAPPFEDMSG HSATYKRIAK VDLKIPSFVP PDARDLISRL LQHNPEKRMS LEQVMRHPWI VKYKDSWTRK SSESS
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required for the spindle checkpoint attachment response during spindle formation, kinetochore microtubule interactions and chromosome segregation during anaphase. Ark1 activity depends upon cut17 function and phosphorylation. Ark1 with bir1 is required for full-scale association with kinetochores and formation of a complex with mad3. Ark1 is also required for phosphorylation of histone H3 that accompanies chromosome condensation and condensin recruitment to mitotic chromatin. Ark1 with pic1 is required for the execution of cytokinesis.
Gene References into Functions
  1. A new role for Aurora B in telomere dispersion and disjunction during fission yeast mitosis. PMID: 25778919
  2. promotes cell division during recovery from TOR-mediated cell cycle arrest by driving spindle pole body recruitment of Polo PMID: 21965528
  3. genetic and cytological analyses suggest that the interplay of chiasmata and kinetochore geometry leads to correct silencing of Aurora B activity at centromeres, providing insight into how chiasmata regulate meiotic chromosome orientation. PMID: 21920317
  4. the Cnd2 subunit of condensin is the target subunit of Aurora-B-like kinase Ark1 and that Ark1-mediated phosphorylation of Cnd2 occurred throughout mitosis PMID: 21540296
Subcellular Location
Nucleus. Cytoplasm, cytoskeleton, spindle. Note=Associates with the elongating spindle during anaphase.
Protein Families
Protein kinase superfamily, Ser/Thr protein kinase family, Aurora subfamily
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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