Manf Antibody

Code CSB-PA896451XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) Manf Polyclonal antibody
Uniprot No.
Target Names
Manf
Alternative Names
Manf antibody; ARP-like antibody; CG7013 antibody; Mesencephalic astrocyte-derived neurotrophic factor homolog antibody; DmMANF antibody; MANF/CDNF-like protein antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) Manf protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Required during the maturation of the embryonic nervous system for maintenance of neuronal and cuticular connectivity. Essential for maintenance of dopaminergic neurons and dopamine levels.
Gene References into Functions
  1. Study employed the Drosophila genetics to screen for potential interaction partners of Drosophila Manf (DmManf) in vivo; show that DmManf plays a role in the development of Drosophila wing. PMID: 28578657
  2. These data suggest a role for Manf in the regulation of Drosophila unfolded protein response. PMID: 26975047
  3. DmMANF expression in the adult brain is much wider than in the embryonic and larval stages. It is expressed in both glia and neurons including dopaminergic neurons. PMID: 25917377
  4. study offers further insights into the functional conservation between invertebrate and mammalian MANF/CDNF proteins and reveals the importance of the C-terminal domain for MANF activity in vivo PMID: 24019940
  5. role of MANF in multicellular organism development PMID: 22494833
  6. DmMANF is an invertebrate neurotrophic factor supporting dopaminergic neurons. PMID: 19164766

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Subcellular Location
Secreted.
Protein Families
ARMET family
Tissue Specificity
In stage 12, expression is seen in the stomodeum and the salivary gland primordia, and weakly in the mesoderm. From stage 13 onward, expression is in Garland cells, the outer wall of the proventriculus and salivary glands. Punctuate expression is also in
Database Links

KEGG: dme:Dmel_CG7013

STRING: 7227.FBpp0082735

UniGene: Dm.3726

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