Recombinant Drosophila melanogaster Mesencephalic astrocyte-derived neurotrophic factor homolog (Manf)

Code CSB-YP896451DLU
MSDS
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Source Yeast
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Code CSB-EP896451DLU
MSDS
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Source E.coli
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Code CSB-EP896451DLU-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896451DLU
MSDS
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Source Baculovirus
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Code CSB-MP896451DLU
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
Manf
Uniprot No.
Alternative Names
Manf; ARP-like; CG7013; Mesencephalic astrocyte-derived neurotrophic factor homolog; DmMANF; MANF/CDNF-like protein
Species
Drosophila melanogaster (Fruit fly)
Expression Region
23-173
Target Protein Sequence
LKEEDCEV CVKTVRRFAD SLDDSTKKDY KQIETAFKKF CKAQKNKEHR FCYYLGGLEE SATGILNELS KPLSWSMPAE KICEKLKKKD AQICDLRYEK QIDLNSVDLK KLKVRDLKKI LNDWDESCDG CLEKGDFIKR IEELKPKYSR SEL
Protein Length
Full Length of Mature Protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Required during the maturation of the embryonic nervous system for maintenance of neuronal and cuticular connectivity. Essential for maintenance of dopaminergic neurons and dopamine levels.
Gene References into Functions
  1. Study employed the Drosophila genetics to screen for potential interaction partners of Drosophila Manf (DmManf) in vivo; show that DmManf plays a role in the development of Drosophila wing. PMID: 28578657
  2. These data suggest a role for Manf in the regulation of Drosophila unfolded protein response. PMID: 26975047
  3. DmMANF expression in the adult brain is much wider than in the embryonic and larval stages. It is expressed in both glia and neurons including dopaminergic neurons. PMID: 25917377
  4. study offers further insights into the functional conservation between invertebrate and mammalian MANF/CDNF proteins and reveals the importance of the C-terminal domain for MANF activity in vivo PMID: 24019940
  5. role of MANF in multicellular organism development PMID: 22494833
  6. DmMANF is an invertebrate neurotrophic factor supporting dopaminergic neurons. PMID: 19164766

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Subcellular Location
Secreted.
Protein Families
ARMET family
Tissue Specificity
In stage 12, expression is seen in the stomodeum and the salivary gland primordia, and weakly in the mesoderm. From stage 13 onward, expression is in Garland cells, the outer wall of the proventriculus and salivary glands. Punctuate expression is also in
Database Links

KEGG: dme:Dmel_CG7013

STRING: 7227.FBpp0082735

UniGene: Dm.3726

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