ARR1 Antibody

Code CSB-PA850050XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ARR1 Polyclonal antibody
Uniprot No.
Target Names
ARR1
Alternative Names
ARR1 antibody; At3g16857 antibody; K20I9.9 antibody; MUH15.1 antibody; Two-component response regulator ARR1 antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ARR1 protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)
Usage
For Research Use Only. Not for use in diagnostic or therapeutic procedures.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Regulates SHY2 by binding to its promoter. Involved in the root-meristem size determination through the regulation of cell differentiation.
Gene References into Functions
  1. The expression signals of AG were detected in the initiating carpel primordia and regenerating carpels, and co-localized with those of two Type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), ARR1 and ARR10. PMID: 29186581
  2. Data suggest that shoot removal induces the quantitative and qualitative development of chloroplasts in roots, with cytokinin signaling via RESPONSE REGULATOR (ARR)-ARR1 and ARR12 functioning downstream of wounding signaling to up-regulate genes associated with chloroplast development. PMID: 28193764
  3. SCR mediates ARR1 transcriptional suppression via the RGA protein. PMID: 26848984
  4. An arr1,10,12 triple mutant had more drought tolerance due to enhanced cell membrane integrity, anthocyanin synthesis, abscissic acid hypersensitivity, and reduced stomatal aperture. Dehydration and ABA repressed all 3 genes. RR1 is the most critical. PMID: 26884175
  5. Cytokinin signaling increases the abundance of ARR1, a ubiquitously expressed type-B response regulators (RRB), by preventing its degradation by the 26S proteasome. PMID: 24617630
  6. Data indicate that one of the immediate early cytokinin response genes is ARR6, which has been shown to be a direct target gene of ARR1, ARR2 and other type-B ARRs. PMID: 23620480
  7. ARR1 mediates cold signal via AHP2 (Arabidopsis histidine kinase 2), AHP3, or AHP5. PMID: 23124324
  8. A nitrosomimetic mutation of AHP1 causes reduced phosphorylation of AHP1 and ARR1, thereby resulting in a compromised cytokinin response. PMID: 23443557
  9. arr1-1 mutation clearly affected the primary response in at least 17 genes, meaning that they respond primarily to cytokinins through the function of ARR1 PMID: 17202182
  10. Suppression of pleiotropic cytokinin activities by a dominant repressor version of a B-type ARR indicates that this protein family is involved in mediating most, if not all, of the cytokinin activities. PMID: 18502977
  11. ARR1 activates SHY2 gene, repressor of auxin signaling that negatively regulates PIN auxin transport facilitator genes: cytokinin causes auxin redistribution, prompting differentiation; auxin mediates degradation of SHY2, sustaining cell division PMID: 19039136

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Subcellular Location
Nucleus.
Protein Families
ARR family, Type-B subfamily
Tissue Specificity
Detected in the whole plant. Expressed at the root transition zone.
Database Links

KEGG: ath:AT3G16857

STRING: 3702.AT3G16857.2

UniGene: At.183

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