Recombinant Arabidopsis thaliana Two-component response regulator ARR1 (ARR1), partial

Code CSB-YP850050DOA
MSDS
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Source Yeast
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Code CSB-EP850050DOA
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Source E.coli
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Code CSB-EP850050DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP850050DOA
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Source Baculovirus
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Code CSB-MP850050DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ARR1
Uniprot No.
Alternative Names
ARR1; At3g16857; K20I9.9; MUH15.1; Two-component response regulator ARR1
Species
Arabidopsis thaliana (Mouse-ear cress)
Protein Length
Partial
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

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Target Background

Function
Transcriptional activator that binds specifically to the DNA sequence 5'-[AG]GATT-3'. Functions as a response regulator involved in His-to-Asp phosphorelay signal transduction system. Phosphorylation of the Asp residue in the receiver domain activates the ability of the protein to promote the transcription of target genes. Could directly activate some type-A response regulators in response to cytokinins. Regulates SHY2 by binding to its promoter. Involved in the root-meristem size determination through the regulation of cell differentiation.
Gene References into Functions
  1. The expression signals of AG were detected in the initiating carpel primordia and regenerating carpels, and co-localized with those of two Type-B ARABIDOPSIS RESPONSE REGULATORs (ARRs), ARR1 and ARR10. PMID: 29186581
  2. Data suggest that shoot removal induces the quantitative and qualitative development of chloroplasts in roots, with cytokinin signaling via RESPONSE REGULATOR (ARR)-ARR1 and ARR12 functioning downstream of wounding signaling to up-regulate genes associated with chloroplast development. PMID: 28193764
  3. SCR mediates ARR1 transcriptional suppression via the RGA protein. PMID: 26848984
  4. An arr1,10,12 triple mutant had more drought tolerance due to enhanced cell membrane integrity, anthocyanin synthesis, abscissic acid hypersensitivity, and reduced stomatal aperture. Dehydration and ABA repressed all 3 genes. RR1 is the most critical. PMID: 26884175
  5. Cytokinin signaling increases the abundance of ARR1, a ubiquitously expressed type-B response regulators (RRB), by preventing its degradation by the 26S proteasome. PMID: 24617630
  6. Data indicate that one of the immediate early cytokinin response genes is ARR6, which has been shown to be a direct target gene of ARR1, ARR2 and other type-B ARRs. PMID: 23620480
  7. ARR1 mediates cold signal via AHP2 (Arabidopsis histidine kinase 2), AHP3, or AHP5. PMID: 23124324
  8. A nitrosomimetic mutation of AHP1 causes reduced phosphorylation of AHP1 and ARR1, thereby resulting in a compromised cytokinin response. PMID: 23443557
  9. arr1-1 mutation clearly affected the primary response in at least 17 genes, meaning that they respond primarily to cytokinins through the function of ARR1 PMID: 17202182
  10. Suppression of pleiotropic cytokinin activities by a dominant repressor version of a B-type ARR indicates that this protein family is involved in mediating most, if not all, of the cytokinin activities. PMID: 18502977
  11. ARR1 activates SHY2 gene, repressor of auxin signaling that negatively regulates PIN auxin transport facilitator genes: cytokinin causes auxin redistribution, prompting differentiation; auxin mediates degradation of SHY2, sustaining cell division PMID: 19039136

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Subcellular Location
Nucleus.
Protein Families
ARR family, Type-B subfamily
Tissue Specificity
Detected in the whole plant. Expressed at the root transition zone.
Database Links

KEGG: ath:AT3G16857

STRING: 3702.AT3G16857.2

UniGene: At.183

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