asf1 Antibody

Code CSB-PA896044XA01DLU
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Product Details

Full Product Name
Rabbit anti-Drosophila melanogaster (Fruit fly) asf1 Polyclonal antibody
Uniprot No.
Target Names
asf1
Alternative Names
asf1 antibody; CG9383Histone chaperone asf1 antibody; Anti-silencing function protein 1 antibody; Replication-coupling assembly factor subunit ASF1 antibody; RCAF subunit ASF1 antibody; dASF1 antibody
Raised in
Rabbit
Species Reactivity
Drosophila melanogaster (Fruit fly)
Immunogen
Recombinant Drosophila melanogaster (Fruit fly) asf1 protein
Immunogen Species
Drosophila melanogaster (Fruit fly)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Plays a role in the formation of silent heterochromatin.
Gene References into Functions
  1. ASF1 is critically required to load H3.3-H4 dimers on the HIRA complex prior to histone deposition on paternal DNA. This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell. In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in asf1 knocked-down embryos. PMID: 29751847
  2. ASF1 cellular levels are tightly controlled by distinct pathways and provide a molecular mechanism for post-translational regulation of dASF1 and hASF1a by TLK kinases. PMID: 20016786
  3. dASF1 has a direct role in modifying chromatin structure during DNA replication and this function of dASF1 is important for the processivity of the replication machinery. PMID: 16396992
  4. Asf1 is directed via interactions with sequence-specific complexes to mediate silencing of specific target genes. PMID: 17925233
Subcellular Location
Nucleus. Chromosome.
Protein Families
ASF1 family
Database Links

KEGG: dme:Dmel_CG9383

STRING: 7227.FBpp0074715

UniGene: Dm.2521

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