Recombinant Drosophila melanogaster Histone chaperone asf1 (asf1)

Code CSB-YP896044DLU
MSDS
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Source Yeast
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Code CSB-EP896044DLU
MSDS
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Source E.coli
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Code CSB-EP896044DLU-B
MSDS
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP896044DLU
MSDS
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Source Baculovirus
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Code CSB-MP896044DLU
MSDS
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
asf1
Uniprot No.
Alternative Names
asf1; CG9383Histone chaperone asf1; Anti-silencing function protein 1; Replication-coupling assembly factor subunit ASF1; RCAF subunit ASF1; dASF1
Species
Drosophila melanogaster (Fruit fly)
Expression Region
1-218
Target Protein Sequence
MAKVHITNVV VLDNPSSFFN PFQFELTFEC IEELKEDLEW KMIYVGSAES EEHDQVLDTI YVGPVPEGRH IFVFQADPPD VSKIPEPDAV GVTIVLLTCS YRGQEFVRVG YYVNNDYADP EMRENPPTKP LFEKLTRNIL ASKPRVTRFK INWDYGHING NGNGVENGHQ DEMATDGPST SEAASAVIHP EDDNSLAMPM ENGIKALNEN SNSLAMEC
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose.
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Histone chaperone that facilitates histone deposition and histone exchange and removal during nucleosome assembly and disassembly. Cooperates with chromatin assembly factor 1 (CAF-1) to promote replication-dependent chromatin assembly. Plays a role in the formation of silent heterochromatin.
Gene References into Functions
  1. ASF1 is critically required to load H3.3-H4 dimers on the HIRA complex prior to histone deposition on paternal DNA. This separation of tasks could optimize the rapid assembly of paternal chromatin within the gigantic volume of the egg cell. In contrast, ASF1 is surprisingly dispensable for the amplification of cleavage nuclei, although chromatin integrity is likely compromised in asf1 knocked-down embryos. PMID: 29751847
  2. ASF1 cellular levels are tightly controlled by distinct pathways and provide a molecular mechanism for post-translational regulation of dASF1 and hASF1a by TLK kinases. PMID: 20016786
  3. dASF1 has a direct role in modifying chromatin structure during DNA replication and this function of dASF1 is important for the processivity of the replication machinery. PMID: 16396992
  4. Asf1 is directed via interactions with sequence-specific complexes to mediate silencing of specific target genes. PMID: 17925233
Subcellular Location
Nucleus. Chromosome.
Protein Families
ASF1 family
Database Links

KEGG: dme:Dmel_CG9383

STRING: 7227.FBpp0074715

UniGene: Dm.2521

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