ATG11 Antibody

Code CSB-PA622493XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG11 Polyclonal antibody
Uniprot No.
Target Names
ATG11
Alternative Names
ATG11 antibody; CVT9 antibody; YPR049C antibody; YP9499.07cAutophagy-related protein 11 antibody; Cytoplasm to vacuole targeting protein 9 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG11 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Involved in cytoplasm to vacuole transport (Cvt), pexophagy, mitophagy and nucleophagy. Recruits mitochondria for their selective degradation via autophagy (mitophagy) during starvation, through its interaction with ATG32. Works as scaffold proteins that recruit ATG proteins to the pre-autophagosome (PAS), the site of vesicle/autophagosome formation. Required for ATG9 anterograde transport from the mitochondria to the PAS. Recruits also the ATG19-prAPE1 complex to the PAS. Required for the Cvt vesicles completion. Plays a significant role in life span extension.
Gene References into Functions
  1. Atg11 plays important roles in low-level nonselective autophagy PMID: 28013049
  2. Atg29 phosphorylation regulates coordination of the Atg17-Atg31-Atg29 complex with the Atg11 scaffold during autophagy initiation. PMID: 23858448
  3. When mitophagy is induced, Atg11 in turn attracts other mitochondrial fission proteins and promotes mitochondrial division. PMID: 23810512
  4. findings suggest that Atg11 plays an integral role in connecting cargo molecules with components of the vesicle-forming machinery PMID: 15659643
  5. Atg11 connecs cargoes, actin and pre-utophagosomal structure (PAS) elements. PMID: 16874092
  6. ATG11 is essential for selective autophagy and mitophagy PMID: 18818209

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Subcellular Location
Preautophagosomal structure membrane; Peripheral membrane protein. Vacuole membrane; Peripheral membrane protein. Note=During pexophagy, accumulates in the vacuolar membrane region, where the peroxisomes contact the vacuole. ATG11 localization is dependent on ATG11-ATG30 interaction.
Protein Families
ATG11 family
Database Links

KEGG: sce:YPR049C

STRING: 4932.YPR049C

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