ATG12 Antibody

Code CSB-PA002283XA01STA
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) ATG12 Polyclonal antibody
Uniprot No.
Target Names
ATG12
Alternative Names
ATG12 antibody; APG12 antibody; SCY_0427Ubiquitin-like protein ATG12 antibody; Autophagy-related protein 12 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain YJM789) (Baker's yeast) ATG12 protein
Immunogen Species
Saccharomyces cerevisiae (strain YJM789) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with ATG5 through a ubiquitin-like conjugating system involving also ATG7 as an E1-like activating enzyme and ATG10 as an E2-like conjugating enzyme, is essential for its function. The ATG12-ATG5 conjugate functions as an E3-like enzyme which is required for lipidation of ATG8 and ATG8 association to the vesicle membranes. ATG12-ATG5 rearranges the ATG3 catalytic center and enhances its E2 activity.
Subcellular Location
Preautophagosomal structure membrane; Peripheral membrane protein.
Protein Families
ATG12 family
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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