atg12 Antibody

Code CSB-PA891592XA01SXV
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Product Details

Full Product Name Rabbit anti-Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) atg12 Polyclonal antibody
Uniprot No. Q9US24
Target Names atg12
Alternative Names atg12 antibody; SPAC1783.06cUbiquitin-like protein ATG12 antibody; Autophagy-related protein 12 antibody
Raised in Rabbit
Species Reactivity Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Immunogen Recombinant Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast) atg12 protein
Immunogen Species Schizosaccharomyces pombe (strain 972 / ATCC 24843) (Fission yeast)
Conjugate Non-conjugated
Clonality Polyclonal
Isotype IgG
Purification Method Protein A/G
Concentration It differs from different batches. Please contact us to confirm it.
Buffer Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form Liquid
Tested Applications ELISA, WB (ensure identification of antigen)
Protocols ELISA Protocol
Troubleshooting and FAQs Antibody FAQs
Storage Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables ① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee ① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time Made-to-order (14-16 weeks)

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Target Background

Function
(From Uniprot)
Ubiquitin-like protein involved in cytoplasm to vacuole transport (Cvt), autophagy vesicles formation, mitophagy, and nucleophagy. Conjugation with atg5 through a ubiquitin-like conjugating system involving also atg7 as an E1-like activating enzyme and atg10 as an E2-like conjugating enzyme, is essential for its function. The atg12-atg5 conjugate functions as an E3-like enzyme which is required for lipidation of atg8 and atg8 association to the vesicle membranes. Plays a role in meiosis and sporulation.
Subcellular Location Cytoplasm. Nucleus. Preautophagosomal structure membrane; Peripheral membrane protein.
Protein Families ATG12 family
Database Links

KEGG: spo:SPAC1783.06c

STRING: 4896.SPAC1783.06c.1

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