ATG3 Antibody

Code CSB-PA334699XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG3 Polyclonal antibody
Uniprot No.
Target Names
ATG3
Alternative Names
ATG3 antibody; APG3 antibody; AUT1 antibody; YNR007C antibody; N2040Autophagy-related protein 3 antibody; Autophagy-related E2-like conjugation enzyme ATG3 antibody
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG3 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
E2 conjugating enzyme required for the cytoplasm to vacuole transport (Cvt) and autophagy. Required for selective autophagic degradation of the nucleus (nucleophagy) as well as for mitophagy which contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Responsible for the E2-like covalent binding of phosphatidylethanolamine to the C-terminal Gly of ATG8. The ATG12-ATG5 conjugate plays a role of an E3 and promotes the transfer of ATG8 from ATG3 to phosphatidylethanolamine (PE). This step is required for the membrane association of ATG8. The formation of the ATG8-phosphatidylethanolamine conjugate is essential for autophagy and for the cytoplasm to vacuole transport (Cvt). The ATG8-PE conjugate mediates tethering between adjacent membranes and stimulates membrane hemifusion, leading to expansion of the autophagosomal membrane during autophagy.
Gene References into Functions
  1. Atg3 is localized to autophagic structures in Saccharomyces cerevisiae. PMID: 25645919
  2. Atg3 is an intrinsically disordered polypeptide and the disorder/order predicted for this protein from its amino acid sequence corresponds to the experimental characteristics PMID: 24879155
  3. Atg3 facilitates cell membrane expansion by ensuring active production of Atg8-PE on the membranes. PMID: 25680528
  4. Autophagic E2 enzymes Atg3 and Atg10 possess insertion regions in the middle of the core fold and may be involved in protein function. PMID: 22993095
  5. the crystallographic structure of Atg3 provides a molecular basis for understanding the unique lipidation reaction that Atg3 carries out PMID: 17227760

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Subcellular Location
Cytoplasm.
Protein Families
ATG3 family
Database Links

KEGG: sce:YNR007C

STRING: 4932.YNR007C

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