ATG32 Antibody

Code CSB-PA340252XA01SVG
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Product Details

Full Product Name
Rabbit anti-Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG32 Polyclonal antibody
Uniprot No.
Target Names
ATG32
Alternative Names
ATG32; ECM17; YIL146C; Autophagy-related protein 32; Extracellular mutant protein 37
Raised in
Rabbit
Species Reactivity
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Immunogen
Recombinant Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast) ATG32 protein
Immunogen Species
Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation.
Gene References into Functions
  1. Results reveal that a posttranslational modification discriminates a form of Atg32 targeting mitochondria for mitophagy from that, which escapes mitophagy by rapid degradation. PMID: 27992522
  2. data implicate Atg32 as a bipartite platform recruiting Atg8 and Atg11 to the mitochondrial surface and forming an initiator complex crucial for mitophagy. PMID: 22308029
  3. Phosphorylation of Serine 114 on Atg32 mediates mitophagy PMID: 21757540
  4. When mitophagy is induced, Atg32 binds to Atg11, an adaptor protein for selective autophagy. PMID: 19736522
  5. Atg32 is a transmembrane receptor that directs autophagosome formation to mitochondria. PMID: 19770589
  6. results suggest that Atg32 (ECM37)acts as a mitophagy-specific receptor and regulates selective degradation of mitochondria PMID: 19619494
  7. YIL146C/ECM37 found essential for mitophagy; designated this autophagy-related (ATG) gene as ATG32; Atg32 confers selectivity for mitochondrial sequestration as a cargo & is necessary for recruitment of this organelle by autophagy machinery for mitophagy PMID: 19619495

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Subcellular Location
Mitochondrion outer membrane; Single-pass membrane protein. Vacuole membrane; Single-pass membrane protein. Preautophagosomal structure membrane; Single-pass membrane protein. Note=Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation.
Protein Families
ATG32 family
Database Links

KEGG: sce:YIL146C

STRING: 4932.YIL146C

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