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Recombinant Saccharomyces cerevisiae Autophagy-related protein 32 (ATG32), partial

Recombinant Saccharomyces cerevisiae Autophagy-related protein 32 (ATG32), partial

Code	CSB-YP340252SVG1
MSDS	MSDS
Size	Pls inquire
Source	Yeast
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Code	CSB-EP340252SVG1
MSDS	MSDS
Size	Pls inquire
Source	E.coli
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Code	CSB-EP340252SVG1-B
MSDS	MSDS
Size	Pls inquire
Source	E.coli
Conjugate	Avi-tag Biotinylated E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code	CSB-BP340252SVG1
MSDS	MSDS
Size	Pls inquire
Source	Baculovirus
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Code	CSB-MP340252SVG1
MSDS	MSDS
Size	Pls inquire
Source	Mammalian cell
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Product Details
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Customer Reviews and Q&A
Target Background

Product Details

Purity

>85% (SDS-PAGE)

Target Names

ATG32

Uniprot No.

P40458

Alternative Names

ATG32; ECM17; YIL146C; Autophagy-related protein 32; Extracellular mutant protein 37

Species

Saccharomyces cerevisiae (strain ATCC 204508 / S288c) (Baker's yeast)

Protein Length

Partial

Tag Info

Tag type will be determined during the manufacturing process.

The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.

Form

Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.

Buffer before Lyophilization

Tris/PBS-based buffer, 6% Trehalose, pH 8.0

Reconstitution

We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.

Troubleshooting and FAQs

Protein FAQs

Storage Condition

Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.

Shelf Life

The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.

Lead Time

Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.

Notes

Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.

Datasheet

Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function

Mitophagy-specific receptor that recruits the autophagic machinery to mitochondria and regulates selective degradation of mitochondria. Mitophagy contributes to regulate mitochondrial quantity and quality by eliminating the mitochondria to a basal level to fulfill cellular energy requirements and preventing excess ROS production. Recruits ATG11 to the surface of mitochondria. Promotes also autophagy-dependent peroxisome degradation.

Gene References into Functions

Results reveal that a posttranslational modification discriminates a form of Atg32 targeting mitochondria for mitophagy from that, which escapes mitophagy by rapid degradation. PMID: 27992522
data implicate Atg32 as a bipartite platform recruiting Atg8 and Atg11 to the mitochondrial surface and forming an initiator complex crucial for mitophagy. PMID: 22308029
Phosphorylation of Serine 114 on Atg32 mediates mitophagy PMID: 21757540
When mitophagy is induced, Atg32 binds to Atg11, an adaptor protein for selective autophagy. PMID: 19736522
Atg32 is a transmembrane receptor that directs autophagosome formation to mitochondria. PMID: 19770589
results suggest that Atg32 (ECM37)acts as a mitophagy-specific receptor and regulates selective degradation of mitochondria PMID: 19619494
YIL146C/ECM37 found essential for mitophagy; designated this autophagy-related (ATG) gene as ATG32; Atg32 confers selectivity for mitochondrial sequestration as a cargo & is necessary for recruitment of this organelle by autophagy machinery for mitophagy PMID: 19619495

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Subcellular Location

Mitochondrion outer membrane; Single-pass membrane protein. Vacuole membrane; Single-pass membrane protein. Preautophagosomal structure membrane; Single-pass membrane protein. Note=Is recruited to the preautophagosomal structure during mitophagy and imported into the vacuole along with mitochondria during starvation.

Protein Families

ATG32 family

Database Links

KEGG: sce:YIL146C

STRING: 4932.YIL146C

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