ATG8A Antibody

Code CSB-PA839689XA01DOA
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Product Details

Full Product Name
Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) ATG8A Polyclonal antibody
Uniprot No.
Target Names
ATG8A
Alternative Names
ATG8A antibody; APG8A antibody; At4g21980 antibody; F1N20.80Autophagy-related protein 8a antibody; Autophagy-related ubiquitin-like modifier ATG8a antibody; AtAPG8a antibody; Protein autophagy 8a antibody
Raised in
Rabbit
Species Reactivity
Arabidopsis thaliana
Immunogen
Recombinant Arabidopsis thaliana ATG8A protein
Immunogen Species
Arabidopsis thaliana (Mouse-ear cress)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Ubiquitin-like modifier involved in autophagosomes formation. May mediate the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton.
Gene References into Functions
  1. Here, we describe how ATG8 can be used to monitor autophagy in Chlamydomonas and Arabidopsis by western blot analysis PMID: 27424752
  2. we describe two common methods used for detection of autophagosomes, staining of autophagosomes with the fluorescent dye monodansylcadaverine, and expression of a fusion between GFP and the autophagosomal membrane protein ATG8. PMID: 27424753
  3. Data indicate that proteasome subunit RPN10 acts as a proteaphagy receptor by binding ubiquitylated proteasomes and autophagy-related protein ATG8. PMID: 26004230
  4. The formation of the ATG12-ATG5 adduct is essential for ATG8-mediated autophagy in plants by promoting ATG8 lipidation. PMID: 20136727
Subcellular Location
Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Cytoplasm, cytoskeleton.
Protein Families
ATG8 family
Tissue Specificity
Constitutively expressed.
Database Links

KEGG: ath:AT4G21980

STRING: 3702.AT4G21980.2

UniGene: At.2106

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