Recombinant Arabidopsis thaliana Autophagy-related protein 8a (ATG8A)

Code CSB-YP839689DOA
MSDS
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Source Yeast
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Code CSB-EP839689DOA
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Source E.coli
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Code CSB-EP839689DOA-B
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Source E.coli
Conjugate Avi-tag Biotinylated
E. coli biotin ligase (BirA) is highly specific in covalently attaching biotin to the 15 amino acid AviTag peptide. This recombinant protein was biotinylated in vivo by AviTag-BirA technology, which method is BriA catalyzes amide linkage between the biotin and the specific lysine of the AviTag.
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Code CSB-BP839689DOA
MSDS
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Source Baculovirus
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Code CSB-MP839689DOA
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Source Mammalian cell
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Product Details

Purity
>85% (SDS-PAGE)
Target Names
ATG8A
Uniprot No.
Alternative Names
ATG8A; APG8A; At4g21980; F1N20.80Autophagy-related protein 8a; Autophagy-related ubiquitin-like modifier ATG8a; AtAPG8a; Protein autophagy 8a
Species
Arabidopsis thaliana (Mouse-ear cress)
Expression Region
1-117
Target Protein Sequence
MAKSSFKISN PLEARMSESS RIREKYPDRI PVIVEKAGQS DVPDIDKKKY LVPADLTVGQ FVYVVRKRIK LGAEKAIFVF VKNTLPPTAA LMSAIYEEHK DEDGFLYMTY SGENTFG
Protein Length
full length protein
Tag Info
Tag type will be determined during the manufacturing process.
The tag type will be determined during production process. If you have specified tag type, please tell us and we will develop the specified tag preferentially.
Form
Lyophilized powder
Note: We will preferentially ship the format that we have in stock, however, if you have any special requirement for the format, please remark your requirement when placing the order, we will prepare according to your demand.
Buffer before Lyophilization
Tris/PBS-based buffer, 6% Trehalose, pH 8.0
Reconstitution
We recommend that this vial be briefly centrifuged prior to opening to bring the contents to the bottom. Please reconstitute protein in deionized sterile water to a concentration of 0.1-1.0 mg/mL.We recommend to add 5-50% of glycerol (final concentration) and aliquot for long-term storage at -20℃/-80℃. Our default final concentration of glycerol is 50%. Customers could use it as reference.
Troubleshooting and FAQs
Storage Condition
Store at -20°C/-80°C upon receipt, aliquoting is necessary for mutiple use. Avoid repeated freeze-thaw cycles.
Shelf Life
The shelf life is related to many factors, storage state, buffer ingredients, storage temperature and the stability of the protein itself.
Generally, the shelf life of liquid form is 6 months at -20°C/-80°C. The shelf life of lyophilized form is 12 months at -20°C/-80°C.
Lead Time
Delivery time may differ from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
Note: All of our proteins are default shipped with normal blue ice packs, if you request to ship with dry ice, please communicate with us in advance and extra fees will be charged.
Notes
Repeated freezing and thawing is not recommended. Store working aliquots at 4°C for up to one week.
Datasheet
Please contact us to get it.

Customer Reviews and Q&A

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Target Background

Function
Ubiquitin-like modifier involved in autophagosomes formation. May mediate the delivery of the autophagosomes to the vacuole via the microtubule cytoskeleton.
Gene References into Functions
  1. Here, we describe how ATG8 can be used to monitor autophagy in Chlamydomonas and Arabidopsis by western blot analysis PMID: 27424752
  2. we describe two common methods used for detection of autophagosomes, staining of autophagosomes with the fluorescent dye monodansylcadaverine, and expression of a fusion between GFP and the autophagosomal membrane protein ATG8. PMID: 27424753
  3. Data indicate that proteasome subunit RPN10 acts as a proteaphagy receptor by binding ubiquitylated proteasomes and autophagy-related protein ATG8. PMID: 26004230
  4. The formation of the ATG12-ATG5 adduct is essential for ATG8-mediated autophagy in plants by promoting ATG8 lipidation. PMID: 20136727
Subcellular Location
Cytoplasmic vesicle, autophagosome membrane; Lipid-anchor. Vacuole membrane; Lipid-anchor. Cytoplasm, cytoskeleton.
Protein Families
ATG8 family
Tissue Specificity
Constitutively expressed.
Database Links

KEGG: ath:AT4G21980

STRING: 3702.AT4G21980.2

UniGene: At.2106

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