axin1 Antibody

Code CSB-PA348954XA01DIL
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Product Details

Full Product Name
Rabbit anti-Danio rerio (Zebrafish) (Brachydanio rerio) axin1 Polyclonal antibody
Uniprot No.
Target Names
axin1
Alternative Names
axin1 antibody; axinAxin-1 antibody; Axis inhibition protein 1 antibody
Raised in
Rabbit
Species Reactivity
Danio rerio (Zebrafish) (Brachydanio rerio)
Immunogen
Recombinant Danio rerio (Zebrafish) (Brachydanio rerio) axin1 protein
Immunogen Species
Danio rerio (Zebrafish) (Brachydanio rerio)
Conjugate
Non-conjugated
Clonality
Polyclonal
Isotype
IgG
Purification Method
Antigen Affinity Purified
Concentration
It differs from different batches. Please contact us to confirm it.
Buffer
Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
Form
Liquid
Tested Applications
ELISA, WB (ensure identification of antigen)
Protocols
Troubleshooting and FAQs
Storage
Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
Value-added Deliverables
① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);
Quality Guarantee
① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;
Lead Time
Made-to-order (14-16 weeks)

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Target Background

Function
Component of the beta-catenin destruction complex required for regulating ctnnb1 levels through phosphorylation and ubiquitination, and modulating Wnt-signaling. Controls dorsoventral patterning via two opposing effects: down-regulates ctnnb1 to inhibit the Wnt signaling pathway and ventralize embryos, but also dorsalizes embryos by activating a Wnt-independent JNK signaling pathway.
Gene References into Functions
  1. beta-catenin1 cannot revert the ich phenotype because it may be under the control of a GSK3beta-independent mechanism that required Axin's RGS domain function. PMID: 25335865
  2. analysis of differential role of Axin RGS domain function in Wnt signaling during anteroposterior patterning and maternal axis formation PMID: 22957046
  3. Wnt/Axin/beta-catenin pathway has a role in ventral CNS development PMID: 15539488
  4. Overactivation of Wnt/Axin1/beta-catenin signaling during late gastrulation leads to bilateral epithalamic expression of Nodal pathway genes independently of lateral plate mesoderm Nodal signaling. PMID: 17678853
  5. A dorsalization pathway that is exerted by Axin/JNK signaling and its inhibitor Aida during vertebrate embryogenesis, is defined. PMID: 17681137
  6. Dab2 stabilizes Axin and attenuates Wnt/beta-catenin signaling by preventing PP1 from binding Axin PMID: 19581931

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Subcellular Location
Cytoplasm. Nucleus. Membrane. Cell membrane.
Database Links
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301-363-4651 (Available 9 a.m. to 5 p.m. CST from Monday to Friday)
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7505 Fannin St., Ste 610, Room 7 (CUBIO Innovation Center), Houston, TX 77054, USA
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