Product Details

Full Product Name

Rabbit anti-Escherichia coli (strain K12) bamB Polyclonal antibody

Uniprot No.

P77774

Target Names

bamB

Alternative Names

bamB antibody; yfgL antibody; b2512 antibody; JW2496Outer membrane protein assembly factor BamB antibody

Raised in

Rabbit

Species Reactivity

Escherichia coli (strain K12)

Immunogen

Recombinant Escherichia coli (strain K12) bamB protein

Immunogen Species

Escherichia coli (strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits. A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins.

Gene References into Functions

The crystal structure shows that BamB has an eight-bladed beta-propeller structure, with four beta-strands making up each blade. PMID: 21168416
BamB could simultaneously bind to BamA and prefolded beta-barrel proteins, thereby enhancing the folding and membrane insertion capability of the Bam complex. PMID: 21236263
A yfgL- negative isogenic mutant showed a marked decrease both in its ability to invade Intestine-407 cells and in the amount of the outer membrane proteins OmpA and OmpC. PMID: 15774871
YfgL is part of a multiprotein complex involved in the assembly of outer membrane beta barrel proteins PMID: 15851036
The data presented here suggest that the yfgL effect on outer membrane proteins also stems from a defective assembly apparatus, leading to aberrant outer membrane protein assembly, except for TolC PMID: 17015657
The periplasmic phosphoquinolipoprotein kinase YfgL plays an important role in radiation-induced DNA strand break repair and homologous recombination in E. coli. PMID: 17630970
Report show that the region of YfgL encompassing L173, L175, and R176 makes direct contact with YaeT and that altering residues D227 and D229 in another region of YfgL from E221 to D229 resulted in defective YaeT bindings. PMID: 18165306

Hide All

Subcellular Location

Cell outer membrane; Lipid-anchor.

Protein Families

BamB family

Database Links

KEGG: ecj:JW2496

STRING: 316385.ECDH10B_2678

Message

Product >> Product Name：
Product >> Code：
Name： *
Email： *
Company/Organization：
Phone：
Since emails may get flagged by spam filters occasionally, we recommend leaving your phone number as a precaution. Your information is safe with us.
Question/Comment： *
Your Region： *
I'd like to receive email newsletters from Cusabio

Yes

No
How do you know CUSABIO?

Email

Distributor

Search engine

Advertisement

Others
CAPTCHA verification：

Code	CSB-PA300609XA01ENV
Size	Enquire
Have Questions?	Leave a Message or Start an on-line Chat

bamB Antibody

Product Details

Related Products

Customer Reviews and Q&A

Target Background