bamB Antibody

Datasheet

Code	CSB-PA300609XA11ENV
Size	US$166
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Image	Western Blot Positive WB detected in: recombinant protein All lanes: bamB Antibody at 1:1000 Secondary Goat polyclonal to rabbit IgG at 1/50000 dilution Predicted band size: 31 kDa Observed band size: 31 kDa
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Target Background

Product Details

Uniprot No.

P77774

Target Names

bamB

Alternative Names

Outer membrane protein assembly factor BamB, bamB, yfgL

Species Reactivity

E.coli

Immunogen

Recombinant E.coli bamB protein (125-386aa)

Immunogen Species

Escherichia coli(strain K12)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Affinity-chromatography

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB

Protocols

ELISA Protocol
Western Blotting(WB) Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Lead Time

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.

Description

The bamB polyclonal antibody is produced by introducing the recombinant E.coli bamB protein (amino acids 125-386) into a rabbit, stimulating the production of antibodies targeting this antigen. A booster injection is administered to enhance antibody production, followed by the collection and purification of serum containing bamB polyclonal antibodies using affinity chromatography. Extensive testing through ELISA and WB assays has verified the bamB antibody's ability to specifically recognize E.coli bamB protein.

The E. coli bamB protein, part of the BamABCDE complex, is an essential component of the bacterial outer membrane protein (OMP) assembly machinery. Its main role is to assist in the correct and efficient assembly of OMPs into the outer membrane of E. coli.

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Target Background

Function

Part of the outer membrane protein assembly complex (Bam), which is involved in assembly and insertion of beta-barrel proteins into the outer membrane. Nonessential member of the complex, which may orient the flexible periplasmic domain of BamA for interaction with other Bam components, chaperones and nascent outer membrane proteins. Efficient substrate folding and insertion into the outer membrane requires all 5 subunits. A lateral gate may open between the first and last strands of the BamA beta-barrel that allows substrate to insert into the outer membrane; comparison of the structures of complete and nearly complete Bam complexes show there is considerable movement of all 5 proteins.

Gene References into Functions

The crystal structure shows that BamB has an eight-bladed beta-propeller structure, with four beta-strands making up each blade. PMID: 21168416
BamB could simultaneously bind to BamA and prefolded beta-barrel proteins, thereby enhancing the folding and membrane insertion capability of the Bam complex. PMID: 21236263
A yfgL- negative isogenic mutant showed a marked decrease both in its ability to invade Intestine-407 cells and in the amount of the outer membrane proteins OmpA and OmpC. PMID: 15774871
YfgL is part of a multiprotein complex involved in the assembly of outer membrane beta barrel proteins PMID: 15851036
The data presented here suggest that the yfgL effect on outer membrane proteins also stems from a defective assembly apparatus, leading to aberrant outer membrane protein assembly, except for TolC PMID: 17015657
The periplasmic phosphoquinolipoprotein kinase YfgL plays an important role in radiation-induced DNA strand break repair and homologous recombination in E. coli. PMID: 17630970
Report show that the region of YfgL encompassing L173, L175, and R176 makes direct contact with YaeT and that altering residues D227 and D229 in another region of YfgL from E221 to D229 resulted in defective YaeT bindings. PMID: 18165306

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Subcellular Location

Cell outer membrane; Lipid-anchor.

Protein Families

BamB family

Database Links

KEGG: ecj:JW2496

STRING: 316385.ECDH10B_2678

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Antibody FAQs What Is an Antibody? How to Choose an Antibody for Scientific Research? The difference between primary antibody and secondary antibod The Meaning of Irregular Antibody Screening Tag Antibodies, Your Good Partner in Protein Research How to Choose the Loading Control Antibodies?

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