Product Details

Full Product Name

Rabbit anti-Arabidopsis thaliana (Mouse-ear cress) BAM3 Polyclonal antibody

Uniprot No.

O65440

Target Names

BAM3

Alternative Names

BAM3 antibody; At4g20270 antibody; F1C12.190Leucine-rich repeat receptor-like serine/threonine-protein kinase BAM3 antibody; EC 2.7.11.1 antibody; Protein BARELY ANY MERISTEM 3 antibody

Raised in

Rabbit

Species Reactivity

Arabidopsis thaliana

Immunogen

Recombinant Arabidopsis thaliana BAM3 protein

Immunogen Species

Arabidopsis thaliana (Mouse-ear cress)

Conjugate

Non-conjugated

Clonality

Polyclonal

Isotype

IgG

Purification Method

Antigen Affinity Purified

Concentration

It differs from different batches. Please contact us to confirm it.

Buffer

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4

Form

Liquid

Tested Applications

ELISA, WB (ensure identification of antigen)

Protocols

ELISA Protocol

Troubleshooting and FAQs

Antibody FAQs

Storage

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.

Value-added Deliverables

① 200ug * antigen (positive control);
② 1ml * Pre-immune serum (negative control);

Quality Guarantee

① Antibody purity can be guaranteed above 90% by SDS-PAGE detection;
② ELISA titer can be guaranteed 1: 64,000;
③ WB validation with antigen can be guaranteed positive;

Lead Time

Made-to-order (14-16 weeks)

Target Background

Function

Necessary for male gametophyte development, as well as ovule specification and function. Required for the development of high-ordered vascular strands within the leaf and a correlated control of leaf shape, size and symmetry. LRR-rich receptor-like kinase (LRR-RLK) involved in the perception of CLE45 peptide ligand which mediates root growth inhibition by repressing protophloem differentiation; this mechanism requires CRN. BRX, BAM3, and CLE45 act together to regulate the transition of protophloem cells from proliferation to differentiation, thus impinging on postembryonic growth capacity of the root meristem. Necessary for CLE45 peptide-triggered accumulation of MAKR5 in developing sieve elements.

Gene References into Functions

BAM3 protein levels remained unchanged under cold stress. Sodium nitroprusside, which releases NO, caused a decline in BAM3.Treatment of recombinant BAM3 with GSNO caused significant, dose-dependent inhibition of BAM3 activity. In vitro BAM3 inhibition was caused by glutathionylation at cysteine 433. The decrease in BAM3 activity under cold stress may be a result of post-translational nitroso- or glutathionylation. PMID: 29309132
study uncovers BAM3 as the CLE45 receptor in the root and biochemically map its peptide binding surface; CRN stabilizes BAM3 expression and is required for BAM3-mediated CLE45 signaling PMID: 28607033
Amino acid composition of BAM1 and BAM3 orthologs reflected differences that are consistent with known adaptations of proteins from heat- and cold-adapted organisms, suggesting that the day- and night-active enzymes have undergone thermal adaptation. PMID: 25293962
Suppression of Arabidopsis protophloem differentiation and root meristem growth by CLE45 requires the receptor-like kinase BAM3. PMID: 23569225

Subcellular Location

Cell membrane; Single-pass type I membrane protein. Endoplasmic reticulum membrane; Single-pass type I membrane protein.

Protein Families

Protein kinase superfamily, Ser/Thr protein kinase family

Tissue Specificity

Expressed in seedlings, roots, leaves, stems, inflorescences, flowers and siliques. In roots, confined to protophloem and sieve element precursor cells.

Database Links

KEGG: ath:AT4G20270

STRING: 3702.AT4G20270.1

UniGene: At.32731

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BAM3 Antibody

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